Application of ribonucleoside vanadyl complex (RVC) for developing a multifunctional tissue preservative solution

• Published in: PloS one Vol. 13; no. 3; p. e0194393
• Main Authors: Shieh, Tzong-Ming, Chen, Chi-Yuan, Hsueh, Chuen, Yu, Cheng-Chia, Chen, Chin-Chuan, Wang, Tong-Hong
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 14.03.2018; Public Library of Science (PLoS)
• Subjects: Analysis; Biological effects; Biological properties; Biological samples; Biology and Life Sciences; Cryopreservation; Deoxyribonucleic acid; DNA; E coli; Escherichia coli; Experiments; Laboratory animals; Medical research; Morphology; Physical Sciences; Proteins; Quality; Research and analysis methods; Reverse transcriptase; Ribonucleic acid; RNA; Staphylococcus aureus; Storage; Tissues; United States > US; Taiwan
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0194393

The quality of biological samples greatly affects the accuracy of scientific results. However, RNA in cryopreserved tissues gradually degrades during storage, leading to errors in the results of subsequent experiments. A suitable sample preservative solution can prolong storage and enhance the research value of samples. Here, we developed a sample preservative solution using the properties of the ribonucleoside vanadyl complex (RVC) and compared its effects on RNA and DNA quality, protein activity, and tissue morphology with the commercially available and widely used RNAlater® Stabilization Solution. The results showed that both the RVC-based preservative solution and RNAlater can effectively delay RNA degradation in tissue samples stored at 4°C or -80°C compared with samples stored without any preservative solution. In contrast to RNAlater, the RVC-based preservative solution did not result in damage to the tissue morphology or a loss of protein activity. Additionally, the RVC-based preservative solution did not affect the RNA and genomic DNA contents of the tissue samples or the results of subsequent experimental analyses. An RVC-based reagent can be used as a multifunctional yet relatively inexpensive tissue preservative solution to provide a comprehensive and cost-effective method for preserving samples for tissue banks.