Characterization of the Erwinia chrysanthemi osmoprotectant transporter gene ousA

• Published in: Journal of Bacteriology Vol. 178; no. 2; pp. 447 - 455
• Main Authors: Gouesbet, G. (CNRS, Rennes, France.), Trautwetter, A, Bonnassie, S, Wu, L.F, Blanco, C
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01.01.1996
• Subjects: ACIDE AMINE; Amino Acid Sequence; Amino Acids, Diamino - metabolism; AMINOACIDOS; ARN MENSAJERO; ARN MESSAGER; BACTERIA; Bacterial Proteins - biosynthesis; Bacterial Proteins - chemistry; Bacterial Proteins - genetics; Bacteriology; BETAINA; BETAINE; Betaine - metabolism; Betaine - pharmacology; Biochemistry; Biochemistry, Molecular Biology; Biodiversity and Ecology; Biological Transport; Carrier Proteins - biosynthesis; Carrier Proteins - chemistry; Carrier Proteins - genetics; Cloning, Molecular; COMPOSE ORGANIQUE; COMPOSICION QUIMICA; COMPOSITION CHIMIQUE; COMPUESTOS ORGANICOS; Culture Media; DNA-Binding Proteins - genetics; DNA-Binding Proteins - physiology; Ecology, environment; Ecosystems; Ecotoxicology; Environmental Sciences; ERWINIA CHRYSANTHEMI; Escherichia coli - genetics; Escherichia coli - growth & development; Escherichia coli Proteins; ESTRUCTURA CELULAR; EXPRESION GENICA; EXPRESSION DES GENES; GENE; Gene Expression Regulation, Bacterial - drug effects; Gene Expression Regulation, Bacterial - physiology; GENES; Genes, Bacterial - genetics; Genetic Complementation Test; GENETICA; GENETIQUE; Genomics; Global Changes; Glucuronidase - biosynthesis; Glucuronidase - genetics; Life Sciences; MEMBRANAS CELULARES; MEMBRANE CELLULAIRE; Membrane Transport Proteins; Molecular biology; Molecular Sequence Data; Molecular Weight; Nuclear Proteins - genetics; Nuclear Proteins - physiology; Osmolar Concentration; Pectobacterium chrysanthemi - genetics; Pectobacterium chrysanthemi - metabolism; Pipecolic Acids - metabolism; PRESION OSMOTICA; PRESSION OSMOTIQUE; PROLINA; PROLINE; Proline - metabolism; PROTEINAS; PROTEINE; Recombinant Fusion Proteins - biosynthesis; SECUENCIA NUCLEICA; Sequence Alignment; Sequence Analysis, DNA; SEQUENCE NUCLEIQUE; Sigma Factor - genetics; STRUCTURE CELLULAIRE; Symporters; Toxicology; Vegetal Biology
• ISSN: 0021-9193; 1098-5530; 1067-8832
• DOI: 10.1128/jb.178.2.447-455.1996

Growth of Erwinia chrysanthemi in media of elevated osmolarity can be achieved by the uptake and accumulation of various osmoprotectants. This study deals with the cloning and sequencing of the ousA gene-encoded osmoprotectant uptake system A from E. chrysanthemi 3937. OusA belongs to the superfamily of solute ion cotransporters. This osmotically inducible system allows the uptake of glycine betaine, proline, ectoine, and pipecolic acid and presents strong similarities in nucleotide sequence and protein function with the proline/betaine porter of Escherichia coli encoded by proP. The control of ous4 expression is clearly different from that of proP. It is induced by osmotic strength and repressed by osmoprotectants. Its expression in E. coli is controlled by H-NS and is rpoS dependent in the exponential phase but unaffected by the stationary phase