A combination of calcium hydroxide and sodium hydrosulphate controls pathogens causing environmental mastitis in recycled manure solids

• Published in: Bioresources and bioprocessing Vol. 11; no. 1; pp. 95 - 12
• Main Authors: Praveen, Selladurai, Kataktalware, Mukund A., Meena, Priyanka, Lavanya, Maharajan, Patoliya, Priyanka, Jeyakumar, Sakthivel, Ravindra, Menon Rekha, Chauhan, Mamta, Ramesha, K. P., Devi, G. Letha, Kastelic, John P., Dhali, Arindam
• Format: Journal Article
• Language: English
• Published: Singapore Springer Nature Singapore 08.10.2024; Springer Nature B.V; SpringerOpen
• Subjects: Bacteria; Biochemical Engineering; Calcium hydroxide; Chemistry; Chemistry and Materials Science; Conditioner combination; Depth; Dewatering; Dung; E coli; Environmental Engineering/Biotechnology; Escherichia coli; Industrial and Production Engineering; Klebsiella; Manure bedding; Manures; Mastitis; Microbial biomass; Microorganisms; Moisture content; Pathogens; Properties; Slaked lime; Sodium; Water content; Conditioner combination; Manure bedding; Properties; Microbial biomass; Depth
• ISSN: 2197-4365; 2197-4365
• DOI: 10.1186/s40643-024-00812-1

Recycled manure solids (RMS) are dried cow dung processed using a manure dewatering machine and subsequently sun-dried to ~ 20% moisture. Benefits of RMS include abundant availability, low cost, and eco-friendliness, but its use as bedding material for cows is hindered by a moisture content that promotes microbial growth. This in vitro study evaluated impacts of calcium hydroxide (CH; 5 and 7.5%) and sodium hydrosulphate (SHS; 6 and 8%), independently and in combinations, at various depths of RMS, on physicochemical and microbial properties. The CH-treated groups had increased pH and reduced moisture on Day 0. Incorporating 7.5% CH + 6% SHS at 15–20 cm, and 7.5% CH + 8% SHS at all depths, effectively suppressed Escherichia coli and Klebsiella spp. Furthermore, a combination of 7.5% CH + 8% SHS at 20 cm inhibited coliform growth, whereas 7.5% CH with 6% SHS inhibited Streptococcus spp . In conclusion, a combination of 7.5% CH with either 6 or 8% SHS at a depth of 15 cm in RMS was particularly effective in controlling environmental mastitis-causing pathogens, specifically E. coli and Klebsiella spp. Graphical Abstract