Genetic Dissection of Barley Morphology and Development

Since the early 20th century, barley (Hordeum vulgare) has been a model for investigating the effects of physical and chemical mutagens and for exploring the potential of mutation breeding in crop improvement. As a consequence, extensive and well-characterized collections of morphological and develo...

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Published inPlant physiology (Bethesda) Vol. 155; no. 2; pp. 617 - 627
Main Authors Druka, Arnis, Franckowiak, Jerome, Lundqvist, Udda, Bonar, Nicola, Alexander, Jill, Houston, Kelly, Radovic, Slobodanka, Shahinnia, Fahimeh, Vendramin, Vera, Morgante, Michele, Stein, Nils, Waugh, Robbie
Format Journal Article
LanguageEnglish
Published Rockville, MD American Society of Plant Biologists 01.02.2011
Subjects
Alleles
backcrossing
Barley
Biological and medical sciences
Chromosome Mapping
crops
Crosses, Genetic
DNA, Plant
DNA, Plant - genetics
Fundamental and applied biological sciences. Psychology
Genes
Genes, Plant
Genetic loci
Genetic mapping
Genetic mutation
genetics
GENOME ANALYSIS
Genomes
Genomics
Genomics - methods
Genotype
Genotypes
growth & development
Hordeum
Hordeum - genetics
Hordeum - growth & development
Hordeum vulgare
methods
mutagenicity
mutants
Mutation
Oryza
Oryza - genetics
Plant physiology and development
Plants
Polymorphism, Single Nucleotide
Rice
single nucleotide polymorphism
Synteny
Barley
Monocotyledones
Hordeum vulgare
Plant physiology
Gramineae
Morphology
Angiospermae
Development
Genetics
Spermatophyta
Cereal crop
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Summary:Since the early 20th century, barley (Hordeum vulgare) has been a model for investigating the effects of physical and chemical mutagens and for exploring the potential of mutation breeding in crop improvement. As a consequence, extensive and well-characterized collections of morphological and developmental mutants have been assembled that represent a valuable resource for exploring a wide range of complex and fundamental biological processes. We constructed a collection of 881 backcrossed lines containing mutant alleles that induce a majority of the morphological and developmental variation described in this species. After genotyping these lines with up to 3,072 single nucleotide polymorphisms, comparison to their recurrent parent defined the genetic location of 426 mutant alleles to chromosomal segments, each representing on average <3% of the barley genetic map. We show how the gene content in these segments can be predicted through conservation of synteny with model cereal genomes, providing a route to rapid gene identification.
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www.plantphysiol.org/cgi/doi/10.1104/pp.110.166249
The online version of this article contains Web-only data.
The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantphysiol.org) is: Arnis Druka (arnis.druka@scri.ac.uk).
Open Access articles can be viewed online without a subscription.
This work was supported by the European Research Area Networks in Plant Genomics (project no. ERAPGFP/06.046A; Genomics-Assisted Dissection of Barley Morphology and Development awarded to R.W., N.S., and M.M. via their respective funding agencies). Development of the Bowman Near Isogenic Lines was possible through long-term funding to J.F. from the American Malting Barley Association, Inc.
ISSN:0032-0889
1532-2548
1532-2548
DOI:10.1104/pp.110.166249