Molecular cloning of a cDNA encoding aldosterone synthase cytochrome P-450 in rat adrenal cortex

• Published in: FEBS letters Vol. 263; no. 2; pp. 299 - 302
• Main Authors: Imai, Michiyo, Shimada, Hideo, Okada, Yukiko, Matsushima-Hibiya, Yuko, Ogishima, Tadashi, Ishimura, Yuzuru
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 24.04.1990; Elsevier
• Subjects: adrenal cortex; Adrenal Cortex - enzymology; Aldosterone; Amino Acid Sequence; Analytical, structural and metabolic biochemistry; Animals; Base Sequence; Biological and medical sciences; bp, base pairs; Cloning, Molecular; Complementary DNA; Cytochrome P-450; Cytochrome P-450 CYP11B2; Cytochrome P-450 Enzyme System - genetics; cytochrome P-450aldo, cytochrome P-450 with aldosterone synthase activity; cytochrome P-45O11β steroid 11-β-hydroxylase; cytochrome P450; DNA - genetics; DOC, 11-deoxycorticosterone; Fundamental and applied biological sciences. Psychology; Hemoproteins; kb, kilobase pairs; Metalloproteins; Molecular cloning; Molecular Sequence Data; Oligonucleotide Probes; Proteins; Rat; Rats; Restriction Mapping; Steroid Hydroxylases - genetics; Steroid-11-hydroxylase; kb, kilobase pairs; DOC, 11-deoxycorticosterone; cytochrome P-45O 11β steroid 11-β-hydroxylase; Complementary DNA; Rat; Cytochrome P-450; Steroid-11-hydroxylase; cytochrome P-450 aldo, cytochrome P-450 with aldosterone synthase activity; Molecular cloning; Aldosterone; bp, base pairs; Hemoprotein; Steroid 11β-monooxygenase; Adrenal glands; Nucleotide sequence; Enzyme; Cytochrome P450; Rodentia; Vertebrata; Mammalia; Isolation; Biosynthesis precursor; Aminoacid sequence
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(90)81398-8

Using an oligonucleotide probe designed on the basis of the N-terminal amino acid sequence of purified rat aldosterone synthase cytochrome P-450 [(1989) J. Biol. Chem. 264 10935] we have isolated from rat adrenal cDNA library a 2687 base pair cDNA that encodes a protein of 500 amino acid residues. The deduced amino acid sequence contained the regions well conserved among all cytochrome P-450s sequenced to date, and also a portion (residues 25–44) which was identical to the N-terminal peptide sequence of rat aldosterone synthase cytochrome P-450. These results indicate that the cDNA encodes a precursor form of rat aldosterone synthase cytochrome P-450.