Establishment of monoclonal antibody against human Apo B-48 and measurement of Apo B-48 in serum by ELISA method

• Published in: Journal of clinical laboratory analysis Vol. 12; no. 5; pp. 289 - 292
• Main Authors: Uchida, Yoshiaki, Kurano, Yoshihiro, Ito, Satoru
• Format: Journal Article
• Language: English
• Published: New York Wiley Subscription Services, Inc., A Wiley Company 1998
• Subjects: Adult; Antibodies, Monoclonal; Apolipoprotein B-48; Apolipoproteins B - blood; Apolipoproteins B - immunology; Apolipoproteins B - isolation & purification; Blotting, Western; chylomicron remnant; Chylomicrons - blood; Chylomicrons - chemistry; Enzyme-Linked Immunosorbent Assay - methods; Fasting - blood; Female; Humans; Male; Original; postprandial lipidemia; Postprandial Period; Western blot analysis

The elevation of chylomicrons and chylomicron remnants in plasma would lead to hyperlipidemia and other complications. Apo B‐48, which is translated and produced in the adult intestine from the same gene as Apo B‐100, is considered to be an essential component of chylomicrons and chylomicron remnant...