Establishment of monoclonal antibody against human Apo B-48 and measurement of Apo B-48 in serum by ELISA method

• Published in: Journal of clinical laboratory analysis Vol. 12; no. 5; pp. 289 - 292
• Main Authors: Uchida, Yoshiaki, Kurano, Yoshihiro, Ito, Satoru
• Format: Journal Article
• Language: English
• Published: New York Wiley Subscription Services, Inc., A Wiley Company 1998
• Subjects: Adult; Antibodies, Monoclonal; Apolipoprotein B-48; Apolipoproteins B - blood; Apolipoproteins B - immunology; Apolipoproteins B - isolation & purification; Blotting, Western; chylomicron remnant; Chylomicrons - blood; Chylomicrons - chemistry; Enzyme-Linked Immunosorbent Assay - methods; Fasting - blood; Female; Humans; Male; Original; postprandial lipidemia; Postprandial Period; Western blot analysis
• ISSN: 0887-8013; 1098-2825
• DOI: 10.1002/(SICI)1098-2825(1998)12:5<289::AID-JCLA7>3.0.CO;2-1

The elevation of chylomicrons and chylomicron remnants in plasma would lead to hyperlipidemia and other complications. Apo B‐48, which is translated and produced in the adult intestine from the same gene as Apo B‐100, is considered to be an essential component of chylomicrons and chylomicron remnants. Using a peptide representing human Apo B‐48 C‐terminal sequence as immunogen, we established a monoclonal antibody, B48‐151, against human Apo B‐48. The specific reactivity for Apo B‐48 of this monoclonal antibody was confirmed using Western blot analysis of human plasma in fractions isolated as chylomicron and VLDL. Then, we developed a simple sandwich ELISA method for the detection of human Apo B‐48 in serum by combining B48‐151 as capturing antibody and HRP‐conjugated‐polyclonal antibodies for Apo B as signaling antibody. The established sandwich ELISA constitutes a simple method to monitor Apo B‐48 level in chylomicrons and chylomicron remnants in human serum. J. Clin. Lab. Anal. 12:289–292, 1998. © 1998 Wiley‐Liss, Inc.