Analysis of the DPC4 Gene in Gastric Carcinoma

• Published in: Cancer science Vol. 88; no. 4; pp. 335 - 339
• Main Authors: Nishizuka, Satoshi, Tamura, Gen, Maesawa, Chihaya, Sakata, Ken, Suzuki, Yasushi, Iwaya, Takeshi, Terashima, Masanori, Saito, Kazuyoshi, Satodate, Ryoichi
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.04.1997; Japanese Cancer Association; John Wiley & Sons, Inc
• Subjects: Biological and medical sciences; Chromosome 18; Chromosome 18q; Chromosome Deletion; Chromosomes, Human, Pair 18; Clonal deletion; Conformation; DNA-Binding Proteins; DPC4; DPC4 gene; Gastric cancer; Gastric carcinoma; Gastroenterology. Liver. Pancreas. Abdomen; Gene deletion; Genes, Tumor Suppressor; Humans; Medical sciences; Mutation; Pancreatic carcinoma; Pancreatic Neoplasms - genetics; Polymerase chain reaction; Polymorphism, Single-Stranded Conformational; Smad4 Protein; Stomach Neoplasms - genetics; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus; Trans-Activators - genetics; Tumor cell lines; Tumor suppressor gene; Tumor suppressor genes; Tumors; Chromosomal aberration; Human; Stomach; Carcinoma; Malignant tumor; Carcinogenesis; Abnormal chromosome E18; Established cell line; Cytogenetics; Deletion; Digestive diseases; Abnormal chromosome; Mutation; Gastric disease; Tumor suppressor gene
• ISSN: 0910-5050; 1347-9032; 1349-7006; 1876-4673
• DOI: 10.1111/j.1349-7006.1997.tb00385.x

Allelic loss on chromosome 18q is involved in the progression of gastric carcinoma. Recently, DPC4 (deleted in pancreatic carcinoma, locus 4), a candidate tumor suppressor gene, has been localized at 18q21.1. This gene is inactivated in nearly one half of pancreatic carcinomas. We tested for DPC4 gene mutations and allelic status at 18q21 in 30 primary gastric carcinomas and 5 gastric carcinoma cell lines. Polymerase chain reaction single‐strand conformation polymorphism and sequencing analyses revealed no DPC4 mutations in any of the primary tumors or cell lines. Homozygous deletion ofDPC4 was observed in only 1 (MKN‐45) of the 5 (20%) cell lines. This suggests that the target gene for loss on 18q is not DPC4. The true tumor suppressor gene, encoded near DPC4, has yet to be identified.