Exercise modulates the insulin-induced translocation of glucose transporters in rat skeletal muscle

• Published in: FEBS letters Vol. 261; no. 2; pp. 256 - 260
• Main Authors: Douen, Andre G., Ramlal, Toolsie, Cartee, Gregory D., Klip, Amira
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 26.02.1990; Elsevier
• Subjects: Animals; Biological and medical sciences; Blotting, Western; Cell Membrane - metabolism; Cell physiology; Cytochalasin B - metabolism; Fundamental and applied biological sciences. Psychology; Glucose - metabolism; Glucose uptake; insulin; Insulin - pharmacology; Insulin action; Male; Membrane and intracellular transports; Molecular and cellular biology; Monosaccharide Transport Proteins - metabolism; Muscles - drug effects; Muscles - metabolism; Physical Exertion - physiology; physical training; Rats; Rats, Inbred Strains; skeletal muscle; Space life sciences; Glucose uptake; Insulin action; Physical exercise; Vertebrata; Mammalia; Rat; Biological transport; Rodentia; Peptide hormone; Plasma membrane; Immunoblotting assay; Glucose; Striated muscle; Insulin
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(90)80566-2

Insulin and acute exercise (45 min of treadmill run) increased glucose uptake into perfused rat hindlimbs 5-fold and 3.2-fold, respectively. Following exercise, insulin treatment resulted in a further increase in glucose uptake. The subcellular distribution of the muscle glucose transporters GLUT-1 and GLUT-4 was determined in plasma membranes and intracellular membranes. Neither exercise nor exercise→ insulin treatment altered the distribution of GLUT-1 transporters in these medmbrane fractions. In contrast, exercise, insulin and exercise→ insulin treatment caused comparable increases in GLUT-4 transporters in the plasma membrane. The results suggest that exercise might limit insulin-induced GLUT-4 recruitment and that following exercise, insulin may alter the intrinsic activity of plasma membrane glucose transporters.