几丁质酶及基因参与水杨酸诱导香蕉枯萎病抗性

由尖孢镰刀菌古巴专化型真菌引起的香蕉枯萎病已成为最具毁灭性的香蕉病害,严重威胁香蕉产业的健康可持续发展.因此对香蕉枯萎病病程相关基因的研究显得尤为重要.比较了Foc4侵染下,香蕉6个病菌应答相关基因的表达;分析了Foc4处理和SA＋Foc4处理的易感病巴西蕉幼苗外部形态特征、几丁质酶活性及几丁质酶基因表达的变化.结果发现Ma-14-3-3c、Ma-14-3-3d、Ma-14-3-3e、Ma-14-3-3f、Ma-Cel和Ma-Chi6个基因都能在转录水平响应Foc4的胁迫;与Foc4处理相比较,SA＋Foc4处理的香蕉幼苗枯萎病抗性增强,根系几丁质酶活性和几丁质酶基因表达水平提高.表明几丁质酶...

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Bibliographic Details
Published in广东农业科学 Vol. 42; no. 7; pp. 64 - 70
Main Author 侯晓婉 徐碧玉 胡伟 颜彦 金志强
Format Journal Article
LanguageChinese
Published 中国热带农业科学院海口实验站,海南海口570102 2015
中国热带农业科学院热带生物技术研究所农业部热带作物生物学与遗传资源利用重点实验室,海南海口571101%中国热带农业科学院热带生物技术研究所农业部热带作物生物学与遗传资源利用重点实验室,海南海口,571101%中国热带农业科学院热带生物技术研究所农业部热带作物生物学与遗传资源利用重点实验室,海南海口571101
海南大学农学院,海南海口570228
Subjects
几丁质酶
尖孢镰刀菌古巴专化型真菌
水杨酸
香蕉
几丁质酶
香蕉
水杨酸
尖孢镰刀菌古巴专化型真菌
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Summary:由尖孢镰刀菌古巴专化型真菌引起的香蕉枯萎病已成为最具毁灭性的香蕉病害,严重威胁香蕉产业的健康可持续发展.因此对香蕉枯萎病病程相关基因的研究显得尤为重要.比较了Foc4侵染下,香蕉6个病菌应答相关基因的表达;分析了Foc4处理和SA＋Foc4处理的易感病巴西蕉幼苗外部形态特征、几丁质酶活性及几丁质酶基因表达的变化.结果发现Ma-14-3-3c、Ma-14-3-3d、Ma-14-3-3e、Ma-14-3-3f、Ma-Cel和Ma-Chi6个基因都能在转录水平响应Foc4的胁迫;与Foc4处理相比较,SA＋Foc4处理的香蕉幼苗枯萎病抗性增强,根系几丁质酶活性和几丁质酶基因表达水平提高.表明几丁质酶等6个基因参与了香蕉枯萎病的生物逆境胁迫应答,同时外源水杨酸处理能够通过诱导香蕉几丁质酶的活性及几丁质酶基因的表达增强香蕉对枯萎病的抗性.
Bibliography:44-1267/S
Fusarium wilt of banana is caused by Fusarium oxysporum f. sp. cubense,has become the most devastating disease of banana and is great harmful to the banana industry＇s healthy and sustainable development. So it is important to study the function of the disease resistance related gene of banana. In this study,we carried out different expression analysis of Ma-14-3-3c,Ma-14-3-3d,Ma-14-3-3e,Ma-14-3-3f,Ma-Cel and Ma-Chi,and compared the difference of the two groups（First group：Banana seedlings only infected with Foc4;Second group：Banana seedlings infected with Foc4 after salicylic acid treatment）in external morphological characteristics,roots＇ chitinase activity and chitinase gene expression. The results showed that compared with the first group,in the second group,the resistance of seedlings to fusarium wiltin creased,the activity of chitinase improved and the expression level of chitinase gene elevated in banana. Therefore,the results demonstrated that chitinase gene,and other five genes,involved in the
ISSN:1004-874X