应用AFLP分子标记研究茶树品种新材料

利用AFLP分子标记技术对28份茶树品种及新材料进行遗传多样性与亲缘关系的研究。结果表明：14对引物组合对样品进行扩增,共扩增得到851条带,多态性条带648条,多态性比率76.10%,其中有5对引物组合的多态性比率高于80%;通过聚类分析,28份供试材料的遗传关系与茶树的原产地有相关性,在相似系数为0.77时分为两个复合组和一个独立组。0302与早白尖5号、0301、0303及0310均从早白尖群体种中单株选育出来,却独立构成这个独立组,表明了其遗传特异性。...

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Bibliographic Details
Published in西南农业学报 Vol. 28; no. 2; pp. 514 - 518
Main Author 侯渝嘉 常亚丽 何桥 唐敏 邬秀红
Format Journal Article
LanguageChinese
Published 重庆市农业科学院茶业研究所,重庆市茶叶工程技术中心,重庆402160%济州国立大学生物科学工程学院,济州,6907563%西南大学,重庆,400716 2015
Subjects
AFLP
茶树品种材料
遗传多样性
茶树品种材料
Genetic diversity
遗传多样性
AFLP
Tea breeds or materials
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Summary:利用AFLP分子标记技术对28份茶树品种及新材料进行遗传多样性与亲缘关系的研究。结果表明：14对引物组合对样品进行扩增,共扩增得到851条带,多态性条带648条,多态性比率76.10%,其中有5对引物组合的多态性比率高于80%;通过聚类分析,28份供试材料的遗传关系与茶树的原产地有相关性,在相似系数为0.77时分为两个复合组和一个独立组。0302与早白尖5号、0301、0303及0310均从早白尖群体种中单株选育出来,却独立构成这个独立组,表明了其遗传特异性。
Bibliography:By AFLP molecular marker,the genetic diversity and phylogenetie relationship were studied for 28 tea breeds or new materials. The results showed： 14 pairs primers with powerful distinctiveness and highly polymorphism were selected for this experiment. Finally, a total of 648 polymorphic bands out of 851 bands were amplified. Percentage of polymorphic loci （p） was 76. 10 %. 5 out of 14 pairs primers displayed more than 80 %, percentage of polymorphic loci ; By cluster analysis that there was a certain correlation between genetic relationship of 28 tea germplasms and their origin places. When Dice coefficient was 0.77, 28 tea cultivars could be classified into 2 complex groups and 1 simple group. Although new 0302, Zaobaijian 5, 0301,0303 and 0310 were all selected and bred from Zaobeijian tea plant group, 0302 individually fell into the simple group, and showed unique genetic characteristic.
51-1213/S
Tea breeds or materials;AFLP;Genetic diversity
HOU Yu-jia, CHANG Ya-li, HE Qiao, TANG Min ,WU Xiu-hong（ 1. Tea Re
ISSN:1001-4829
DOI:10.16213/j.cnki.scjas.2015.02.013