芽孢杆菌C产亚硝酸还原酶的发酵培养基优化及酶学性质研究
通过单因素试验和响应面分析对亚硝酸还原酶产生菌c的发酵条件进行优化，获得最佳发酵培养基为：葡萄糖2．26％，蛋白胨0．2％，NH4C10．12％，K2HPO4·H2O0．2％，MgSO40．075％，KCl0．1％，FeSO40．004％，NaNO20．2％，在此条件下，酶活达到317．82U／mL，比优化前的190．26U／mL提高了67．05％。产生的亚硝酸还原酶的酶学性质表明，该酶被Mn^2＋、Mg^2＋、Na^＋激活，受Fe^2＋、EDTA抑制；最适温度和pH值分别为30℃和7．4；当温度低于50℃、pH值在7．0～8．0时，酶能保持较高的活性。与现有文献报道的筛选细菌相比较，该菌株亚...
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| Published in | 广东农业科学 Vol. 42; no. 16; pp. 71 - 78 |
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| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
华南理工大学生物科学与工程学院,广东广州,510006
2015
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1004-874X |
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| Summary: | 通过单因素试验和响应面分析对亚硝酸还原酶产生菌c的发酵条件进行优化，获得最佳发酵培养基为：葡萄糖2．26％，蛋白胨0．2％，NH4C10．12％，K2HPO4·H2O0．2％，MgSO40．075％，KCl0．1％，FeSO40．004％，NaNO20．2％，在此条件下，酶活达到317．82U／mL，比优化前的190．26U／mL提高了67．05％。产生的亚硝酸还原酶的酶学性质表明，该酶被Mn^2＋、Mg^2＋、Na^＋激活，受Fe^2＋、EDTA抑制；最适温度和pH值分别为30℃和7．4；当温度低于50℃、pH值在7．0～8．0时，酶能保持较高的活性。与现有文献报道的筛选细菌相比较，该菌株亚硝酸还原酶产量处于较高水平。 |
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| Bibliography: | 44-1267/S HAN Qian, CUI Tang-bing （ School ofBioscience ＆ Bioengineering, South China University of Technology, Guangzhou 510006, China ） nitrite reductase; fermentation medium optimization; enzymatic properties; response surface analysis The fermentation conditions of nitrite reductase produced by Bacillus sp. C were optimized by single factor experiment and response surface analysis. The optimal fermentation conditions were as follows： 2.26% glucose, 0.2% peptone, NH4Cl 0.12%, K2HPO4 ·H2O 0.2%, MgSO4 0.075%, KC1 0.1%, FeSO4 0.004%, NaNO2 0.2%. In these conditions, the enzyme activity reached 317.82 U/mL, increased by 67.05% compared with the activity before optimization with 190.26 U/mL. The results showed that the nitrite reductase was activated by Mn^2＋, Mg^2＋, Na^＋ and inhibited by Fe^2＋ and EDTA; the optimal temperature was 30℃, the optimal pH was 7.4; the enzyme preserved higher activity at the temperature below 50℃ and pH 7.0-8.0. Compared with the existing literature, the yield of nitrite reductase prod |
| ISSN: | 1004-874X |