玉米WRKY转录因子非生物胁迫的表达分析

WRKY蛋白是一类植物特异的转录因子家族,在植物响应病害及非生物胁迫中起重要作用。通过生物信息学方法,从玉米基因组中得到3个WRKY家族基因序列（ZmWRKY14-like、ZmWRKY25-like、ZmWRKY62-like）,预测表明3个蛋白都定位于细胞核。荧光定量PCR分析表明,3个基因在玉米的不同器官中都有表达,但具有组织表达特异性。ZmWRKY14-like和ZmWRKY62-like在幼果中的表达量均显著高于在其他组织中的表达量,ZmWRKY25-like基因在叶、穗丝和幼果中的表达量高于在其他组织中的表达量。盐胁迫下,ZmWRKY25-like基因呈上调表达,ZmWRKY62-...

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Bibliographic Details
Published in广东农业科学 Vol. 44; no. 1; pp. 15 - 22
Main Author 决登伟 桑雪莲 舒波 刘丽琴 王一承 石胜友
Format Journal Article
LanguageChinese
Published 中国热带农业科学院南亚热带作物研究所/农业部热带果树生物学重点实验室,广东湛江,524091 2017
Subjects
WRKY转录因子
低温
干旱
玉米
盐胁迫
低温
WRKY转录因子
玉米
干旱
盐胁迫
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Summary:WRKY蛋白是一类植物特异的转录因子家族,在植物响应病害及非生物胁迫中起重要作用。通过生物信息学方法,从玉米基因组中得到3个WRKY家族基因序列（ZmWRKY14-like、ZmWRKY25-like、ZmWRKY62-like）,预测表明3个蛋白都定位于细胞核。荧光定量PCR分析表明,3个基因在玉米的不同器官中都有表达,但具有组织表达特异性。ZmWRKY14-like和ZmWRKY62-like在幼果中的表达量均显著高于在其他组织中的表达量,ZmWRKY25-like基因在叶、穗丝和幼果中的表达量高于在其他组织中的表达量。盐胁迫下,ZmWRKY25-like基因呈上调表达,ZmWRKY62-like基因呈下调表达;干旱胁迫下,ZmWRKY62-like基因出现下调表达;低温胁迫下,ZmWRKY25-like基因呈上调表达。结果表明,ZmWRKY25-like可能参与了植物对盐和低温胁迫的响应,ZmWRKY62-like基因则可能参与了植物对盐和干旱胁迫的响应。
Bibliography:maize ; WRKY TFs ; drought stress ; salt stress ; low temperature stress
44-1267/S
WRKY proteins are large family of plant-specific transcription factors （ TFs ）, play essential roles in regulating plant responses to pathogens and abiotic stress. In this study, we identified three WRKY transcription factor （ TF ） genes （ZmWRKY14-1ike, ZmWRKY25-1ike, ZmWRKY62-1ike ） in maize by using bioinformatics method. Plant-mPLoe predicted that these three proteins were all localized in nucleus. Real-time qPCR assay showed that ZmWRKY14-like, ZmWRKY25-1ike, ZmWRKY62-1ike expressed in all tested maize tissues, but had differential expression. The expression levels of ZmWRKY14-like and ZmWRKY62-1ike in young fruit were significantly higher than those in other tissues, while the expression level of ZmWRKY25-like gene in leaf, spike and young fruit was higher than that in other tissues. The expression levels of three genes under different abiotic stress were also analyzed by real-time qPCR. The expression of ZmWRKY25-1ike was u
ISSN:1004-874X
DOI:10.16768/j.issn.1004-874X.2017.01.003