胰腺癌细胞纳米探针的磁共振荧光双模态成像及抗肿瘤效果观察

目的：探讨QDs＠ Gd‐RGD纳米探针用于MIA Ⅱ胰腺癌细胞磁共振（MRI）荧光双模态成像的效果及其对胰腺癌细胞的抑制作用。方法：构建QDs＠ Gd‐RGD纳米探针，将溶于磷酸盐缓冲液（PBS ）的QDs＠ Gd‐RGD纳米探针（QDs＠ Gd‐RGD组）、Gd（Gd组）分别与M IA Ⅱ胰腺癌细胞共孵育1 h ，Control组不做处理，然后用荧光相差显微镜及1．5 T M RI仪观察3组的成像特点。3组M IA Ⅱ胰腺癌细胞培养24 h后，观察细胞形态、增殖、克隆、迁移、细胞周期、凋亡的变化。结果：荧光相差显微镜下观察发现，QDs＠ Gd‐RGD纳米探针分布于MIA Ⅱ胰腺癌细胞膜，而...

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Bibliographic Details
Published in中国临床医学 Vol. 23; no. 4; pp. 428 - 432
Main Author 刘峰君 叶雯 何欣源 施裕新
Format Journal Article
LanguageChinese
Published 上海市公共卫生临床中心,上海,201508 2016
Subjects
M
RI荧光双模态成像
抗肿瘤治疗
胰腺癌细胞
靶向诊断
anti-tumor therapy
pancreatic cancer cell
MRI and fluorescent dual-modality imaging
抗肿瘤治疗
胰腺癌细胞
targeted diagnosis
QDs Gd-RGD
靶向诊断
M RI荧光双模态成像
Online AccessGet full text
ISSN1008-6358
DOI10.12025/j.issn.1008-6358.2016.20160671

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Summary:目的：探讨QDs＠ Gd‐RGD纳米探针用于MIA Ⅱ胰腺癌细胞磁共振（MRI）荧光双模态成像的效果及其对胰腺癌细胞的抑制作用。方法：构建QDs＠ Gd‐RGD纳米探针，将溶于磷酸盐缓冲液（PBS ）的QDs＠ Gd‐RGD纳米探针（QDs＠ Gd‐RGD组）、Gd（Gd组）分别与M IA Ⅱ胰腺癌细胞共孵育1 h ，Control组不做处理，然后用荧光相差显微镜及1．5 T M RI仪观察3组的成像特点。3组M IA Ⅱ胰腺癌细胞培养24 h后，观察细胞形态、增殖、克隆、迁移、细胞周期、凋亡的变化。结果：荧光相差显微镜下观察发现，QDs＠ Gd‐RGD纳米探针分布于MIA Ⅱ胰腺癌细胞膜，而Gd组及Control组细胞膜及细胞内未观察到荧光分布；1．5 T MRI成像显示，QDs＠ Gd‐RGD组信号强度明显高于Gd组、Control组（P＜0．05）。QDs＠ Gd‐RGD组MIAⅡ胰腺癌细胞失去典型铺路石样形态、细胞核碎裂、细胞质空泡化，细胞克隆能力明显下降，细胞增殖抑制，细胞迁移能力减弱，细胞周期明显被阻滞，细胞凋亡增加（P＜0．05）。结论：QDs＠ Gd‐RGD纳米探针用于MIA Ⅱ胰腺癌细胞MRI荧光双模态成像的效果较好；QDs＠ Gd‐RGD纳米探针体外对M IA Ⅱ胰腺癌细胞有一定的杀伤作用。
Bibliography:31-1794/R
LIU Feng-jun, YE Wen, HE Xin-yuan, SHI Yu-xin （Shanghai Public Health Clinical Center, Shanghai 201508, China）
QDs Gd-RGD;pancreatic cancer cell;QDs Gd-RGD;MRI and fluorescent dual-modality imaging;targeted diagnosis;anti-tumor therapy
Objective：To investigate the effects of QDs@ Gd‐RGD nano probe on fluorescent and MRI dual‐modality imaging of M IA Ⅱ pancreatic cancer cells and its role in inhibiting pancreatic cancer cells .Methods：QDs@ Gd‐RGD nano probe was prepared .QDs@ Gd‐RGD nano probe （QDs@ Gd‐RGD group） and Gd （Gd group） were dissolved in phosphate buffer solution （PBS） and incubated with MIA Ⅱ pancreatic cancer cells for 1 h ,and no treatment was given to the control group .And then imaging characteristics of the 3 groups were observed by fluorescence phase contrast microscope and 1 .5 T MRI observation .After 3 groups of MIA Ⅱ pancreatic cancer cells were cultured for 24 h ,cell morphology ,proliferation , cloning ,migration ,cycle and apoptosis of MIA Ⅱ pancreatic cancer cells were observed
ISSN:1008-6358
DOI:10.12025/j.issn.1008-6358.2016.20160671