猪伪狂犬病病毒双重PCR鉴别方法的建立

根据GenBank已发表的猪伪狂犬病毒（PRV）的gB、gE基因序列，分别设计了2对特异性引物，建立了一种鉴别诊断猪伪狂犬病病毒野毒与疫苗毒的双重PCR检测方法。应用该方法可从野毒基因组中扩增出与预期大小相符的2条特异性条带，分别为122bp（gE）和246bp（gB）；从疫苗株基因组中仅扩增出1条特异性条带，为246bp（gB）。结果显示，该方法灵敏度高、特异性强，适用于PRV的临床诊断和流行病学调查。...

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Published in广东农业科学 Vol. 42; no. 17; pp. 130 - 133
Main Author 李艳 李段 向蓉 刘燕玲 蒋智勇 张乐宜 蔡汝健 李春玲
Format Journal Article
LanguageChinese
Published 广东省农科院动物卫生研究所/广东省畜禽疫病防治研究重点实验室/广东省兽医公共卫生公共实验室,广东广州,510640 2015
Subjects
伪狂犬病病毒
双重PCR
鉴定
鉴定
伪狂犬病病毒
双重PCR
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Summary:根据GenBank已发表的猪伪狂犬病毒（PRV）的gB、gE基因序列，分别设计了2对特异性引物，建立了一种鉴别诊断猪伪狂犬病病毒野毒与疫苗毒的双重PCR检测方法。应用该方法可从野毒基因组中扩增出与预期大小相符的2条特异性条带，分别为122bp（gE）和246bp（gB）；从疫苗株基因组中仅扩增出1条特异性条带，为246bp（gB）。结果显示，该方法灵敏度高、特异性强，适用于PRV的临床诊断和流行病学调查。
Bibliography:44-1267/S
Based on the gB and gE genes of pseudorabies virus （ PRV ）, two pairs of special primers were designed, duplex PCR for distinguishing the wild strain and vaccine strain of PRV was established. By this duplex PCR assay, two specific fragments of 122 bp （gE） and 246 bp （gB） were simultaneously amplified from field isolate DNA, only a 246 bp （ gB ） fragment was amplified from vaccine strain DNA. The results indicated that the method was specific, sensitive, and could be used for rapid diagnosis and epidemiological investigation of PRV infection.
pseudorabies virus; duplex PCR ; identification
LI Yan, LI Duan, XIANG Rong, LIU Yan-ling, JIANG Zhi-yong, ZHANG Le-yi, CAI Ru-jian, LI Chun-ling （ Institute of Animal Health, Guangdong Academy of Agricultural Sciences/Guangdong Open Laboratory of Veterinary Public Health/Guangdong Laboratory for Animal Diseases, Guangzhou 510640, China ）
ISSN:1004-874X