Gene Expression Patterns in Human Embryonic Stem Cells and Human Pluripotent Germ Cell Tumors

Remarkably little is known about the transcriptional profiles of human embryonic stem (ES) cells or the molecular mechanisms that underlie their pluripotency. To identify commonalties among the transcriptional profiles of different human pluripotent cells and to search for clues into the genesis of...

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Published inProceedings of the National Academy of Sciences - PNAS Vol. 100; no. 23; pp. 13350 - 13355
Main Authors Sperger, Jamie M., Chen, Xin, Draper, Jonathan S., Antosiewicz, Jessica E., Chon, Chris H., Jones, Sunita B., Brooks, James D., Andrews, Peter W., Brown, Patrick O., Thomson, James A.
Format Journal Article
LanguageEnglish
Published United States National Academy of Sciences 11.11.2003
National Acad Sciences
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Summary:Remarkably little is known about the transcriptional profiles of human embryonic stem (ES) cells or the molecular mechanisms that underlie their pluripotency. To identify commonalties among the transcriptional profiles of different human pluripotent cells and to search for clues into the genesis of human germ cell tumors, we compared the expression profiles of human ES cell lines, human germ cell tumor cell lines and tumor samples, somatic cell lines, and testicular tissue samples by using cDNA microarray analysis. Hierarchical cluster analysis of gene expression profiles showed that the five independent human ES cell lines clustered tightly together, reflecting highly similar expression profiles. The gene expression patterns of human ES cell lines showed many similarities with the human embryonal carcinoma cell samples and more distantly with the seminoma samples. We identified 895 genes that were expressed at significantly greater levels in human ES and embryonal carcinoma cell lines than in control samples. These genes are candidates for involvement in the maintenance of a pluripotent, undifferentiated phenotype.
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Communicated by Neal L. First, University of Wisconsin, Madison, WI, September 8, 2003
Abbreviations: EC, embryonal carcinoma; ES, embryonic stem; GCT, germ cell tumor; ICM, inner cell mass; PGC, primordial germ cell; SAM, significance analysis of microarrays; YSC, yolk sac carcinoma.
To whom correspondence may be addressed at: 1220 Capitol Court, Madison, WI 53715; e-mail: thomson@primate.wisc.edu (for J.A.T.); or Beckman B400, Stanford, CA 94305-5307; e-mail: patrick.brown@stanford.edu (for P.O.B.).
J.M.S. and X.C. contributed equally to this work.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.2235735100