Distribution and respiratory activity of mycobacteria in household water system of healthy volunteers in Japan

• Published in: PloS one Vol. 9; no. 10; p. e110554
• Main Authors: Ichijo, Tomoaki, Izumi, Yoko, Nakamoto, Sayuri, Yamaguchi, Nobuyasu, Nasu, Masao
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 28.10.2014; Public Library of Science (PLoS)
• Subjects: Acanthamoeba polyphaga; Bacteria; Bacterial Load; Biofilms; Biology and Life Sciences; Contamination; Deoxyribonucleic acid; DNA; Drinking water; Environmental science; Exposure; Family Characteristics; Fluorescence; Healthy Volunteers; Households; Humans; Infections; Infectious diseases; Japan; Laboratories; Methods; Mycobacterium; Mycobacterium - metabolism; Mycobacterium avium; Mycobacterium Infections - epidemiology; Mycobacterium Infections - microbiology; Mycobacterium Infections, Nontuberculous - epidemiology; Mycobacterium Infections, Nontuberculous - microbiology; Mycobacterium intracellulare; Mycobacterium tuberculosis; Nontuberculous Mycobacteria - metabolism; Opportunist infection; Pathogens; Pharmaceutical sciences; Tuberculosis; Water consumption; Water Microbiology; Water use; Japan
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0110554

The primary infectious source of nontuberculous mycobacteria (NTM), which are known as opportunistic pathogens, appears to be environmental exposure, and it is important to reduce the frequency of exposure from environmental sources for preventing NTM infections. In order to achieve this, the distribution and respiratory activity of NTM in the environments must be clarified. In this study, we determined the abundance of mycobacteria and respiratory active mycobacteria in the household water system of healthy volunteers using quantitative PCR and a fluorescent staining method, because household water has been considered as one of the possible infectious sources. We chose healthy volunteer households in order to lessen the effect of possible residential contamination from an infected patient. We evaluated whether each sampling site (bathroom drain, kitchen drain, bath heater pipe and showerhead) have the potential to be the sources of NTM infections. Our results indicated that drains in the bathroom and kitchen sink are the niche for Mycobacterium spp. and M. avium cells were only detected in the bathtub inlet. Both physicochemical and biologic selective pressures may affect the preferred habitat of Mycobacterium spp. Regional differences also appear to exist as demonstrated by the presence (US) or absence (Japan) of Mycobacterium spp. on showerheads. Understanding of the country specific human activities and water usage will help to elucidate the infectious source and route of nontuberculous mycobacterial disease.