Photosynthetic Responses to Heat Treatments at Different Temperatures and following Recovery in Grapevine (Vitis amurensis L.) Leaves

• Published in: PloS one Vol. 6; no. 8; p. e23033
• Main Authors: Luo, Hai-Bo, Ma, Ling, Xi, Hui-Feng, Duan, Wei, Li, Shao-Hua, Loescher, Wayne, Wang, Jun-Fang, Wang, Li-Jun
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 26.08.2011; Public Library of Science (PLoS)
• Subjects: Activation; Agricultural production; Biology; Botany; Carbon; Carbon dioxide; Carbon Dioxide - metabolism; Carbon dioxide concentration; Chlorophyll; Chlorophyll - metabolism; Climate; Conductance; Cyanobacteria; Electron Transport; Electron transport chain; Enzyme Activation; Fluorescence; Fruits; Glycine max; Grapes; Grapevines; Heat; Heat recovery; Heat stress; Heat tolerance; Heat treatment; Heat treatments; High temperature; Hot Temperature; Laboratories; Leaves; Metabolism; Nitrogen; Oryza; Photosynthesis; Photosynthesis - physiology; Photosystem I Protein Complex - metabolism; Photosystem II; Photosystem II Protein Complex - metabolism; Plant biochemistry; Plant Leaves - enzymology; Plant Leaves - physiology; Plant sciences; Plant Stomata - enzymology; Plant Stomata - physiology; Recovery (Medical); Resistance; Ribulose-bisphosphate carboxylase; Ribulose-Bisphosphate Carboxylase - metabolism; Stomata; Stomatal conductance; Stress (Physiology); Studies; Vitis - enzymology; Vitis - physiology; Vitis labrusca; Beijing China; United States > US; China; Michigan
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0023033

The electron transport chain, Rubisco and stomatal conductance are important in photosynthesis. Little is known about their combined responses to heat treatment at different temperatures and following recovery in grapevines (Vitis spp.) which are often grown in climates with high temperatures. The electron transport function of photosystem II, the activation state of Rubisco and the influence of stomatal behavior were investigated in grapevine leaves during heat treatments and following recovery. High temperature treatments included 35, 40 and 45°C, with 25°C as the control and recovery temperature. Heat treatment at 35°C did not significantly (P>0.05) inhibit net photosynthetic rate (P(n)). However, with treatments at 40 and 45°C, P(n) was decreased, accompanied by an increase in substomatal CO(2) concentration (C(i)), decreases in stomatal conductance (g(s)) and the activation state of Rubisco, and inhibition of the donor side and the reaction center of PSII. The acceptor side of PSII was inhibited at 45°C but not at 40°C. When grape leaves recovered following heat treatment, P(n), g(s) and the activation state of Rubisco also increased, and the donor side and the reaction center of PSII recovered. The increase in P(n) during the recovery period following the second 45°C stress was slower than that following the 40°C stress, and these increases corresponded to the donor side of PSII and the activation state of Rubisco. Heat treatment at 35°C did not significantly (P>0.05) influence photosynthesis. The decrease of P(n) in grape leaves exposed to more severe heat stress (40 or 45°C) was mainly attributed to three factors: the activation state of Rubisco, the donor side and the reaction center of PSII. However, the increase of P(n) in grape leaves following heat stress was also associated with a stomatal response. The acceptor side of PSII in grape leaves was responsive but less sensitive to heat stress.