Bacteriolytic Therapy Can Generate a Potent Immune Response against Experimental Tumors
When spores of the anaerobic bacterium Clostridium novyi-NT are systemically injected into animals, they germinate exclusively within the hypoxic regions of cancers. The germinated bacteria destroy adjacent tumor cells but spare a rim of well oxygenated tumor cells that subsequently expand. Surprisi...
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Published in | Proceedings of the National Academy of Sciences - PNAS Vol. 101; no. 42; pp. 15172 - 15177 |
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Main Authors | , , , , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
National Academy of Sciences
19.10.2004
National Acad Sciences |
Subjects | |
Online Access | Get full text |
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Summary: | When spores of the anaerobic bacterium Clostridium novyi-NT are systemically injected into animals, they germinate exclusively within the hypoxic regions of cancers. The germinated bacteria destroy adjacent tumor cells but spare a rim of well oxygenated tumor cells that subsequently expand. Surprisingly, we found that ≈30% of mice treated with such spores were cured of their cancers despite the viable tumor rim initially remaining after spore germination. The mechanism underlying this effect was shown to be immune-mediated, because cured animals rejected a subsequent challenge of the same tumor. Similar effects were observed in rabbits with intrahepatic tumors. It was particularly notable that the induced immune response, when combined with the bacteriolytic effects of C. novyi-NT, could eradicate large established tumors. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 To whom correspondence should be addressed. E-mail: vogelbe@jhmi.edu. Abbreviations: CT, computed tomography; PET, positron emission tomography; FDG, [18F]fluorodeoxyglucose. C.B. and I.C. contributed equally to this work. Contributed by Bert Vogelstein, August 25, 2004 |
ISSN: | 0027-8424 1091-6490 |
DOI: | 10.1073/pnas.0406242101 |