A prominent role for DC-SIGN+ dendritic cells in initiation and dissemination of measles virus infection in non-human primates

Measles virus (MV) is a highly contagious virus that is transmitted by aerosols. During systemic infection, CD150(+) T and B lymphocytes in blood and lymphoid tissues are the main cells infected by pathogenic MV. However, it is unclear which cell types are the primary targets for MV in the lungs and...

Full description

Saved in:
Bibliographic Details
Published inPloS one Vol. 7; no. 12; p. e49573
Main Authors Mesman, Annelies W, de Vries, Rory D, McQuaid, Stephen, Duprex, W Paul, de Swart, Rik L, Geijtenbeek, Teunis B H
Format Journal Article
LanguageEnglish
Published United States Public Library of Science 05.12.2012
Public Library of Science (PLoS)
Subjects
Alveoli
Animals
Antigens
Biology
Bronchoalveolar Lavage Fluid
CD150 antigen
Cell Adhesion Molecules - metabolism
DC-SIGN protein
Dendritic cells
Dendritic Cells - immunology
Dendritic Cells - metabolism
Disseminated infection
Gene expression
HIV
Human immunodeficiency virus
Immunology
Infections
Lectins, C-Type - metabolism
Lungs
Lymph nodes
Lymphatic system
Lymphocytes
Lymphocytes B
Lymphocytes T
Lymphoid tissue
Macaca fascicularis
Measles
Measles - immunology
Medicine
Primates
Proteins
Receptors, Cell Surface - metabolism
Studies
Tissues
Vaccines
Viruses
Online AccessGet full text

Cover

More Information
Summary:Measles virus (MV) is a highly contagious virus that is transmitted by aerosols. During systemic infection, CD150(+) T and B lymphocytes in blood and lymphoid tissues are the main cells infected by pathogenic MV. However, it is unclear which cell types are the primary targets for MV in the lungs and how the virus reaches the lymphoid tissues. In vitro studies have shown that dendritic cell (DC) C-type lectin DC-SIGN captures MV, leading to infection of DCs as well as transmission to lymphocytes. However, evidence of DC-SIGN-mediated transmission in vivo has not been established. Here we identified DC-SIGN(hi) DCs as first target cells in vivo and demonstrate that macaque DC-SIGN functions as an attachment receptor for MV. Notably, DC-SIGN(hi) cells from macaque broncho-alveolar lavage and lymph nodes transmit MV to B lymphocytes, providing in vivo support for an important role for DCs in both initiation and dissemination of MV infection.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Conceived and designed the experiments: AWM RDdV RLdS TBHG. Performed the experiments: AWM RDdV SM. Analyzed the data: AWM RDdV SM WPD RLdS TBHG. Wrote the paper: AWM RDdV WPD RLdS TBHG.
Competing Interests: Dr. Geijtenbeek serves as an editor for PLOS ONE and declares that he does not have any competing interests. This does not alter the authors’ adherence to all the PLOS ONE policies on sharing data and materials, as detailed online in the guide for authors. The authors do not have any potential competing interests.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0049573