Secretor genotype (FUT2 gene) is strongly associated with the composition of Bifidobacteria in the human intestine

• Published in: PloS one Vol. 6; no. 5; p. e20113
• Main Authors: Wacklin, Pirjo, Mäkivuokko, Harri, Alakulppi, Noora, Nikkilä, Janne, Tenkanen, Heli, Räbinä, Jarkko, Partanen, Jukka, Aranko, Kari, Mättö, Jaana
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 19.05.2011; Public Library of Science (PLoS)
• Subjects: Abundance; Analysis; Antigenic determinants; Antigens; Bacteria; Bifidobacterium - isolation & purification; Bioinformatics; Biology; Blood; Blood groups; Deoxyribonucleic acid; Determinants; DNA; Electrophoresis, Polyacrylamide Gel; Enzymes; Epitopes; Fucosyltransferases - genetics; FUT2 gene; Galactoside 2-a-L-fucosyltransferase; Galactoside 2-alpha-L-fucosyltransferase; Genes; Genetic aspects; Genetic research; Genotype; Genotypes; Glycan; Humans; Intestinal microflora; Intestine; Intestines - microbiology; Irritable bowel syndrome; Microbiota (Symbiotic organisms); Mucosa; Mucus; Mutation; Nonsense mutation; Polymerase Chain Reaction; Principal components analysis; Secretions; Studies; Twins
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0020113

Intestinal microbiota plays an important role in human health, and its composition is determined by several factors, such as diet and host genotype. However, thus far it has remained unknown which host genes are determinants for the microbiota composition. We studied the diversity and abundance of dominant bacteria and bifidobacteria from the faecal samples of 71 healthy individuals. In this cohort, 14 were non-secretor individuals and the remainders were secretors. The secretor status is defined by the expression of the ABH and Lewis histo-blood group antigens in the intestinal mucus and other secretions. It is determined by fucosyltransferase 2 enzyme, encoded by the FUT2 gene. Non-functional enzyme resulting from a nonsense mutation in the FUT2 gene leads to the non-secretor phenotype. PCR-DGGE and qPCR methods were applied for the intestinal microbiota analysis. Principal component analysis of bifidobacterial DGGE profiles showed that the samples of non-secretor individuals formed a separate cluster within the secretor samples. Moreover, bifidobacterial diversity (p<0.0001), richness (p<0.0003), and abundance (p<0.05) were significantly reduced in the samples from the non-secretor individuals as compared with those from the secretor individuals. The non-secretor individuals lacked, or were rarely colonized by, several genotypes related to B. bifidum, B. adolescentis and B. catenulatum/pseudocatenulatum. In contrast to bifidobacteria, several bacterial genotypes were more common and the richness (p<0.04) of dominant bacteria as detected by PCR-DGGE was higher in the non-secretor individuals than in the secretor individuals. We showed that the diversity and composition of the human bifidobacterial population is strongly associated with the histo-blood group ABH secretor/non-secretor status, which consequently appears to be one of the host genetic determinants for the composition of the intestinal microbiota. This association can be explained by the difference between the secretor and non-secretor individuals in their expression of ABH and Lewis glycan epitopes in the mucosa.