The crowding dynamics of the motor protein kinesin-II

• Published in: PloS one Vol. 15; no. 2; p. e0228930
• Main Authors: Kushwaha, Vandana S, Acar, Seyda, Miedema, Daniël M, Denisov, Dmitry V, Schall, Peter, Peterman, Erwin J G
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 13.02.2020; Public Library of Science (PLoS)
• Subjects: Amino acids; Astronomy; Biology and Life Sciences; Chemoreception; Cilia; Cloning; Cooperativity; Correlation analysis; Crowding; E coli; Fluorescence; Fluorescence microscopy; Kinesin; Mathematical models; Molecular motors; Motility; Parameters; Physics; Plasmids; Polypeptides; Protein transport; Proteins; Research and Analysis Methods; Traffic congestion; Transport; Netherlands
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0228930

Intraflagellar transport (IFT) in C. elegans chemosensory cilia is an example of functional coordination and cooperation of two motor proteins with distinct motility properties operating together in large groups to transport cargoes: a fast and processive homodimeric kinesin-2, OSM-3, and a slow and less processive heterotrimeric kinesin-2, kinesin-II. To study the mechanism of the collective dynamics of kinesin-II of C. elegans cilia in an in vitro system, we used Total Internal Reflection Fluorescence microscopy to image the motility of truncated, heterodimeric kinesin-II constructs at high motor densities. Using an analysis technique based on correlation of the fluorescence intensities, we extracted quantitative motor parameters, such as motor density, velocity and average run length, from the image. Our experiments and analyses show that kinesin-II motility parameters are far less affected by (self) crowding than OSM-3. Our observations are supported by numerical calculations based on the TASEP-LK model (Totally Asymmetric Simple Exclusion Process-Langmuir Kinetics). From a comparison of data and modelling of OSM-3 and kinesin-II, a general picture emerges of the collective dynamics of the kinesin motors driving IFT in C. elegans chemosensory cilia and the way the motors deal with crowding.