Panmicrobial oligonucleotide array for diagnosis of infectious diseases

To facilitate rapid, unbiased, differential diagnosis of infectious diseases, we designed GreeneChipPm, a panmicrobial microarray comprising 29,455 sixty-mer oligonucleotide probes for vertebrate viruses, bacteria, fungi, and parasites. Methods for nucleic acid preparation, random primed PCR amplifi...

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Published inEmerging infectious diseases Vol. 13; no. 1; pp. 73 - 81
Main Authors Palacios, Gustavo, Quan, Phenix-lan, Jabado, Omar J, Conlan, Sean, Hirschberg, David L, Liu, Yang, Zhai, Junhui, Renwick, Neil, Hui, Jeffrey, Hegyi, Hedi, Grolla, Allen, Strong, James E, Towner, Jonathan S, Geisbert, Thomas W, Jahrling, Peter B, Büchen-Osmond, Cornelia, Ellerbrok, Heinz, Sanchez-Seco, Maria Paz, Lussier, Yves, Formenty, Pierre, Nichol, M Stuart T, Feldmann, Heinz, Briese, Thomas, Lipkin, W Ian
Format Journal Article
LanguageEnglish
Published United States U.S. National Center for Infectious Diseases 01.01.2007
Centers for Disease Control and Prevention
Subjects
Communicable diseases
Communicable Diseases - diagnosis
Communicable Diseases - virology
Diagnosis
differential diagnosis
Disease Outbreaks
DNA microarrays
Fatal Outcome
Genetic aspects
Humans
Malaria
Malaria, Falciparum - diagnosis
microarray analytical devices
Oligonucleotide Array Sequence Analysis - instrumentation
Oligonucleotide Array Sequence Analysis - methods
oligonucleotide microarrays
Phylogeny
Sensitivity and Specificity
viral hemorrhagic fever
Virus Diseases - diagnosis
Virus Diseases - virology
Canada
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Summary:To facilitate rapid, unbiased, differential diagnosis of infectious diseases, we designed GreeneChipPm, a panmicrobial microarray comprising 29,455 sixty-mer oligonucleotide probes for vertebrate viruses, bacteria, fungi, and parasites. Methods for nucleic acid preparation, random primed PCR amplification, and labeling were optimized to allow the sensitivity required for application with nucleic acid extracted from clinical materials and cultured isolates. Analysis of nasopharyngeal aspirates, blood, urine, and tissue from persons with various infectious diseases confirmed the presence of viruses and bacteria identified by other methods, and implicated Plasmodium falciparum in an unexplained fatal case of hemorrhagic feverlike disease during the Marburg hemorrhagic fever outbreak in Angola in 2004-2005.
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ISSN:1080-6040
1080-6059
DOI:10.3201/eid1301.060837