Detection of miRNAs with a nanopore single-molecule counter

miRNAs are short noncoding RNA molecules that are important in regulating gene expression. Due to the correlation of their expression levels and various diseases, miRNAs are being investigated as potential biomarkers for molecular diagnostics. The fast-growing miRNA exploration demands rapid, accura...

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Bibliographic Details
Published inExpert review of molecular diagnostics Vol. 12; no. 6; pp. 573 - 584
Main Authors Gu, Li-Qun, Wanunu, Meni, Wang, Michael X, McReynolds, Larry, Wang, Yong
Format Journal Article
LanguageEnglish
Published England Taylor & Francis 01.07.2012
Expert Reviews Ltd
Informa Healthcare
Subjects
Biological markers
biomarker
biomarkers
biosensor
diagnostics
Gene expression
Gene Expression Profiling - methods
Gene Expression Regulation
Humans
lung cancer
MicroRNA
MicroRNAs - analysis
miRNA
nanopore
Nanopores
Nanotechnology - methods
noninvasive detection
PCR
Physiological aspects
Reviews
RNA
United States
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Summary:miRNAs are short noncoding RNA molecules that are important in regulating gene expression. Due to the correlation of their expression levels and various diseases, miRNAs are being investigated as potential biomarkers for molecular diagnostics. The fast-growing miRNA exploration demands rapid, accurate, low-cost miRNA detection technologies. This article will focus on two platforms of nanopore single-molecule approach that can quantitatively measure miRNA levels in samples from tissue and cancer patient plasma. Both nanopore methods are sensitive and specific, and do not need labeling, enzymatic reaction or amplification. In the next 5 years, the nanopore-based miRNA techniques will be improved and validated for noninvasive and early diagnosis of diseases.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
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ISSN:1473-7159
1744-8352
DOI:10.1586/erm.12.58