Influence of concentration, time and method of application of citric acid and sodium citrate in root conditioning

• Published in: Journal of applied oral science Vol. 20; no. 3; pp. 376 - 383
• Main Authors: Cavassim, Rodrigo, Leite, Fábio Renato Manzolli, Zandim, Daniela Leal, Dantas, Andrea Abi Rached, Rached, Ricardo Samih Georges Abi, Sampaio, José Eduardo Cezar
• Format: Journal Article
• Language: English
• Published: Brazil Faculdade de Odontologia de Bauru da Universidade de São Paulo 01.05.2012; Faculdade De Odontologia De Bauru - USP; University of São Paulo
• Subjects: Acid Etching, Dental - methods; Chelating Agents - administration & dosage; Chelating Agents - analysis; Citrates - administration & dosage; Citrates - analysis; Citric Acid - administration & dosage; Citric Acid - analysis; Collagen; Collagen - drug effects; Dental Cavity Preparation - instrumentation; Dental Cavity Preparation - methods; Dental scaling; Dentistry; DENTISTRY, ORAL SURGERY & MEDICINE; Humans; Microscopy, Electron, Scanning; Original; Periodontics; Smear Layer; Sodium Citrate; Time Factors; Tooth Root - drug effects; Periodontics; Smear layer; Dental scaling; Collagen
• ISSN: 1678-7757; 1678-7765; 1678-7765; 1678-7757
• DOI: 10.1590/S1678-77572012000300014

The aim of this study was to establish the parameters of concentration, time and mode of application of citric acid and sodium citrate in relation to root conditioning. A total of 495 samples were obtained and equally distributed among 11 groups (5 for testing different concentrations of citric acid, 5 for testing different concentrations of sodium citrate and 1 control group). After laboratorial processing, the samples were analyzed under scanning electron microscopy. A previously calibrated and blind examiner evaluated micrographs of the samples. Non-parametric statistical analysis was performed to analyze the data obtained. Brushing 25% citric acid for 3 min, promoted greater exposure of collagen fibers in comparison with the brushing of 1% citric acid for 1 minute and its topical application at 1% for 3 min. Sodium citrate exposed collagen fibers in a few number of samples. Despite the lack of statistical significance, better results for collagen exposure were obtained with brushing application of 25% citric acid for 3 min than with other application parameter. Sodium citrate produced a few number of samples with collagen exposure, so it is not indicated for root conditioning.