Delivery of pDNA to the Lung by Lipopolyplexes Using N-Lauroylsarcosine and Effect on the Pulmonary Fibrosis

• Published in: Pharmaceutics Vol. 13; no. 11; p. 1983
• Main Authors: Kurosaki, Tomoaki, Kanda, Hiroki, Hashizume, Junya, Sato, Kayoko, Harasawa, Hitomi, Nakamura, Tadahiro, Sasaki, Hitoshi, Kodama, Yukinobu
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 22.11.2021; MDPI
• Subjects: Ethanol; Experiments; gene delivery; Gene expression; Gene therapy; Growth factors; Lipids; Lungs; N-lauroylsarcosine; nanoparticles; Polymers; Pulmonary fibrosis; shRNA; St Louis Missouri; United States > US; Japan
• ISSN: 1999-4923; 1999-4923
• DOI: 10.3390/pharmaceutics13111983

In a previous study, we constructed a lung-targeting lipopolyplex containing polyethyleneimine (PEI), 1,2-di-O-octadecenyl-3-trimethylammonium propane (DOTMA), and N-lauroylsarcosine (LS). The lipopolyplex exhibited an extremely high gene expression in the lung after intravenous administration. Here, we optimized the lipopolyplex and used it to deliver a TGF-β1 shRNA to treat refractory pulmonary fibrosis. We constructed several lipopolyplexes with pDNA, various cationic polymers, cationic lipids, and LS to select the most effective formulation. Then, the pDNA encoding shRNA against mouse TGF-β1 was encapsulated in the lipopolyplex and injected into mice with bleomycin-induced pulmonary fibrosis. After optimizing the lipopolyplex, dendrigraft poly-L-lysine (DGL) and DOTMA were selected as the appropriate cationic polymer and lipid, respectively. The lipopolyplex was constructed with a pDNA, DGL, DOTMA, and LS charge ratio of 1:2:2:4 showed the highest gene expression. After intravenous administration of the lipopolyplex, the highest gene expression was observed in the lung. In the in vitro experiment, the lipopolyplex delivered pDNA into the cells via endocytosis. As a result, the lipopolyplex containing pDNA encoding TGF-β1 shRNA significantly decreased hydroxyproline in the pulmonary fibrosis model mice. We have successfully inhibited pulmonary fibrosis using a novel lung-targeting lipopolyplex.