Potential inhibitory effects of d-allose, a rare sugar, on liver preneoplastic lesion development in F344 rat medium-term bioassay

• Published in: Journal of bioscience and bioengineering Vol. 105; no. 5; pp. 545 - 553
• Main Authors: Yokohira, Masanao, Hosokawa, Kyoko, Yamakawa, Keiko, Saoo, Kousuke, Matsuda, Yoko, Zeng, Yu, Kuno, Toshiya, Imaida, Katsumi
• Format: Journal Article
• Language: English
• Published: Amsterdarm Elsevier B.V 01.05.2008; Osaka, Japan: Society for Bioscience and Bioengineering, Japan; Amsterdam, the Netherlands: Distributed outside Japan by Elsevier Science; Elsevier Science; Elsevier Limited
• Subjects: Animals; Biological and medical sciences; Biotechnology; choline deficient; Cytokines - immunology; d-allose; Fundamental and applied biological sciences. Psychology; Glucose - administration & dosage; liver carcinogenesis; Liver Neoplasms - immunology; Liver Neoplasms - prevention & control; Male; medium-term bioassay; oxygen radicals; Precancerous Conditions - immunology; Precancerous Conditions - prevention & control; rare sugar; Rats; Rats, Inbred F344; Treatment Outcome; liver carcinogenesis; d-allose; medium-term bioassay; rare sugar; oxygen radicals; choline deficient; Oxygen; Bioassay; Rat; Liver; Rodentia; Carcinogenesis; Vertebrata; Mammalia; Choline; D-allose; Inhibition; Lesion; Sugar
• ISSN: 1389-1723; 1347-4421
• DOI: 10.1263/jbb.105.545

d-Allose, the C-3 epimer of d-glucose, is a monosaccharide present in minute quantities in nature and a rare sugar. The effects of d-allose on diethyl nitrosamine (DEN)-induced hepatocarcinogenesis were examined in male F344 rats by a rat medium-term bioassay based on the two-step model of hepatocarcinogenesis (experiment 1). In addition, a DNA microarray analysis was employed to clarify possible mechanisms of action of d-allose (experiment 2). The antioxidation potential of d-allose solution itself or of serum in rats treated with d-allose was also examined directly by measuring Cu +-reducing antioxidation power (experiment 3). Furthermore, to investigate the effects of d-allose in vivo under conditions of oxidative stress, it was administered with a choline-deficient, l-amino acid-defined diet (CDAA) in the medium-term liver carcinogenesis bioassay (experiment 4). Experiment 1 demonstrated no effects of d-allose on the development of glutathione S-transferase placental form (GST-P) positive foci in the liver. From DNA microarray analysis, several mRNA markers were found to be altered with functions related to apoptosis and cell proliferation (experiment 2), although d-allose itself and serum in vivo exhibited no antioxidation power directly (experiment 3). When d-allose was administered with the CDAA diet, decreases in the area and number of GST-P positive foci were noted with P values of 0.158 for area (%) and 0.061 for number (/cm 2) (experiment 4). These results suggest the potential inhibitory effect of d-allose on liver carcinogenesis, particularly under oxidative stress conditions.