Staphylococcus sciuri exfoliative toxin C (ExhC) is a necrosis-inducer for mammalian cells
Staphylococcus sciuri (S. sciuri) is a rare pathogen in humans, but it can cause a wide array of human infections. Recently a S. sciuri isolate (HBXX06) was reported to cause fatal exudative epidermitis (EE) in piglets and thus considered as a potential zoonotic agent. To investigate the pathogenici...
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Published in | PloS one Vol. 6; no. 7; p. e23145 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
United States
Public Library of Science
29.07.2011
Public Library of Science (PLoS) |
Subjects | |
Online Access | Get full text |
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Summary: | Staphylococcus sciuri (S. sciuri) is a rare pathogen in humans, but it can cause a wide array of human infections. Recently a S. sciuri isolate (HBXX06) was reported to cause fatal exudative epidermitis (EE) in piglets and thus considered as a potential zoonotic agent. To investigate the pathogenicity of this bacterium, we cloned exfoliative toxin C (ExhC), a major toxin of the S. sciuri isolate and performed functional analysis of the recombinant ExhC-his (rExhC) protein using in vitro cell cultures and newborn mice as models. We found that rExhC could induce necrosis in multiple cell lines and peritoneal macrophages as well as skin lesions in newborn mice, and that the rExhC-induced necrosis in cells or skin lesions in newborn mice could be completely abolished if amino acids 79-128 of rExhC were deleted or blocked with a monoclonal antibody (3E4), indicating aa 79-128 portion as an essential necrosis-inducing domain. This information contributes to further understandings of the mechanisms underlying S. sciuri infection. |
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Bibliography: | Conceived and designed the experiments: SJZ HL. Performed the experiments: HL YW LD. Analyzed the data: SJZ HL YW. Wrote the paper: SJZ HL. |
ISSN: | 1932-6203 1932-6203 |
DOI: | 10.1371/journal.pone.0023145 |