Sensitivity and specificity of serum soluble interleukin-2 receptor for diagnosing sarcoidosis in a population of patients suspected of sarcoidosis

• Published in: PloS one Vol. 14; no. 10; p. e0223897
• Main Authors: Eurelings, Laura E M, Miedema, Jelle R, Dalm, Virgil A S H, van Daele, Paul L A, van Hagen, P Martin, van Laar, Jan A M, Dik, Willem A
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 17.10.2019; Public Library of Science (PLoS)
• Subjects: Adult; Angiotensin; Biology and Life Sciences; Biomarkers; Biomarkers - blood; Biopsy; Cohort Studies; Cytokines; Departments; Diagnosis; Diagnostic systems; Diagnostic tests; Disease; Early Diagnosis; Enzymes; Female; Histology; Humans; Immunology; Interleukin 2; Internal medicine; Laboratories; Laboratory tests; Male; Medical diagnosis; Medical imaging; Medicine; Medicine and Health Sciences; Middle Aged; Peptidyl-dipeptidase A; Peptidyl-Dipeptidase A - blood; Receptors, Interleukin-2 - blood; ROC Curve; Sarcoidosis; Sarcoidosis - diagnosis; Sarcoidosis - metabolism; Sensitivity; Sensitivity and Specificity; Tomography; Netherlands
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0223897

The soluble interleukin 2 receptor (sIL-2R) has been proposed as a marker of disease activity in patients with sarcoidosis. However, no studies have evaluated whether serum sIL-2R measurement is of use in establishing the diagnosis of sarcoidosis in patients who are suspected of sarcoidosis among other diseases. A cohort study was conducted, consisting of new patients who visited the immunology outpatient clinic and whose serum sIL-2R levels were available before a definitive diagnosis was established between February 2011 and February 2016. All patients underwent standard diagnostic testing for sarcoidosis (e.g. laboratory tests, radiographic and/or nuclear imaging and/or affected site biopsy). This resulted either in the diagnosis of sarcoidosis or the exclusion of sarcoidosis with the diagnosis of another disease. Results of sIL-2R and angiotensin-converting enzyme (ACE) levels, radiographic and nuclear imaging and histology results were collected and definitive diagnoses were recorded. Sensitivity, specificity, the concordance statistic from the receiver operating characteristic curve and Youden's Index were calculated to assess the performance of sIL-2R in the diagnosis of sarcoidosis and were compared to ACE, currently one of the most used diagnostic biomarkers in the diagnosis of sarcoidosis. In total 983 patients were screened for inclusion, of which 189 patients met the inclusion criteria. A total of 101 patients were diagnosed with sarcoidosis after diagnostic workup, of whom 79 were biopsy-proven. In 88 patients a diagnosis other than sarcoidosis was made. The sensitivity and specificity of serum soluble interleukin 2 receptor levels to detect sarcoidosis were 88% and 85%. The sensitivity and specificity of ACE were 62% and 76%. Receiver operating characteristic curve analysis revealed that sIL-2R receptor is superior to ACE (p<0.0001). Serum sIL-2R is a sensitive biomarker and superior to ACE in establishing the diagnosis of sarcoidosis and can be used to rule out sarcoidosis in patients suspected of sarcoidosis.