Nuclear magnetic resonance spectroscopy to determine the micellar cholesterol in human bile

The cholesterol of gallstones comes from the vesicular rather than the micellar phase of bile. Progress in this field has been limited because conventional analytical methods disturb the distribution of cholesterol between the two phases. The resonance of the cholesterol C 6 proton occurs at a chemi...

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Published inFEBS letters Vol. 300; no. 1; pp. 30 - 32
Main Authors Ellul, J.P.M., Murphy, G.M., Parkes, H.G., Slapa, R.Z., Dowling, R.H.
Format Journal Article
LanguageEnglish
Published Amsterdam Elsevier B.V 23.03.1992
Elsevier
Subjects
bile
Bile - chemistry
Biliary cholesterol
Biological and medical sciences
cholesterol
Cholesterol - chemistry
Cholesterol NMR
Digestive system
Humans
Investigative techniques, diagnostic techniques (general aspects)
levels
Magnetic Resonance Spectroscopy
man
Medical sciences
Micelle
Micelles
N.M.R
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Vesicle
Vesicle
Micelle
Cholesterol NMR
Biliary cholesterol
Human
Biliary stone
Analysis method
NMR spectrometry
Cholesterol
Bile
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Summary:The cholesterol of gallstones comes from the vesicular rather than the micellar phase of bile. Progress in this field has been limited because conventional analytical methods disturb the distribution of cholesterol between the two phases. The resonance of the cholesterol C 6 proton occurs at a chemical shift of 5.4 ppm, to be shown by 2D NMR to be specific for biliary cholesterol, and arises only from the micellar mode. Thus integration of C 6 proton resonance peak area provides a direct non-invasive determination of the cholesterol distribution in human bile.
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ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(92)80158-D