Di-myo-inositol-1, 1′-phosphate: A new inositol phosphate isolated from Pyrococcus woesei

• Published in: FEBS letters Vol. 306; no. 2; pp. 239 - 242
• Main Authors: Scholz, Stefan, Sonnenbichler, Johann, Schäfer, Wolfram, Hensel, Reinhard
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 20.07.1992; Elsevier
• Subjects: Analytical, structural and metabolic biochemistry; Archaea; Archaea - chemistry; Archaea - metabolism; Biological and medical sciences; Chromatography, Thin Layer; Enzyme Stability; Fundamental and applied biological sciences. Psychology; Glyceraldehyde-3-Phosphate Dehydrogenases - metabolism; Inositol Phosphates - isolation & purification; Inositol Phosphates - metabolism; Intermediary metabolites. Miscellaneous; Intracellular ion concentration; Magnetic Resonance Spectroscopy; Molecular Structure; Other biological molecules; Potassium - metabolism; Protein thermostabilization; Pyrococcus woesei; Temperature; Thormophile; Protein thermostabilization; Intracellular ion concentration; Archaea; Thormophile; Proteins; Molecular structure; Archaeobacteria; Bacteria; Isolation; Inositol phosphate; Biological activity; Thermal stability
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(92)81008-A

A new inositol derivative could be isolated from the Archaeum Pyrococcus woesei and identified as di-myo-inositol-1,1′-phosphate by 1H, 31P NMR spectroscopy, mass spectrometry and thin layer chromatograpy. In P. woesel, this inositol phosphate represents the dominant counterion of K + which ranges from 500 to 600 mM. The role of the potassium salt of di-myo-inositol-1,1′-phosphate as thermostabilizer is discussed.