Predicted sequence of the host-protective immunogen of infectious bursal disease virus

• Published in: FEBS letters Vol. 201; no. 1; pp. 143 - 146
• Main Authors: Hudson, Peter J., McKern, Neil M., Fahey, Kevin J., Azad, Ahmed A.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 26.05.1986; Elsevier
• Subjects: Amino Acid Sequence; Base Sequence; Biological and medical sciences; chickens; DNA - genetics; DNA, Recombinant; Fundamental and applied biological sciences. Psychology; GENBANK, the nucleotide sequence database of the Los Alamos National Laboratory, Los Alamos, USA; Genes, Viral; IBDV, infectious bursal disease virus; Infectious bursal disease virus; Infectious bursal disease virus - genetics; infectious bursitis virus; Mab, monoclonal antibody; Microbiology; monoclonal antibodies; Morphology, structure, chemical composition, physicochemical properties; NBRF, the protein sequence database of the National Biomedical Research Foundation, Washington, USA; Nucleotide sequence; Primary structure; Reoviridae - genetics; RNA, Viral - genetics; Vaccine; Viral Proteins - genetics; Viral Proteins - immunology; viral RNA; Viral Vaccines; Virology; Nucleotide sequence; Infectious bursal disease virus; IBDV, infectious bursal disease virus; Mab, monoclonal antibody; Primary structure; GENBANK, the nucleotide sequence database of the Los Alamos National Laboratory, Los Alamos, USA; Vaccine; viral RNA; NBRF, the protein sequence database of the National Biomedical Research Foundation, Washington, USA; Virus; Immunogenicity; RNA
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(86)80587-7

The genome of Australian strain 002-73 of infectious bursal disease virus (IBDV) has been cloned as cDNA fragments into an expression library based on pUR plasmid vectors. Recombinant colonies were selected with a monoclonal antibody specific for the 32-kDa host-protective immunogen of IBDV and fully characterized by nucleotide sequence analysis. The amino acid sequence of several tryptic peptides derived from the native 32-kDa structural protein has confirmed the coding region assignment and nucleotide sequence data. We believe this to be the first published sequence of a birnavirus-encoded protein and these data may provide the basis for an effective subunit vaccine against IBDV