DNA sequence analysis of diuron-resistant mutations in the mitochondrial cytochrome b gene of Saccharomyces cerevisiae

• Published in: FEBS letters Vol. 208; no. 2; pp. 208 - 210
• Main Authors: di Rago, J.-P., Perea, X., Colson, A.-M.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 24.11.1986; Elsevier
• Subjects: (Saccharomyces cerevisiae) Diuron resistance Mitochondrial cytochrome b split gene DNA sequence analysis Primer extension Mutational alteration; (Saccharomyces cerevisiae) Diuron resistanceMitochondrial cytochrome b split geneDNA sequence analysisPrimer extensionMutational alteration; Amino Acid Sequence; Base Sequence; Biological and medical sciences; Classical genetics, quantitative genetics, hybrids; Cytochrome b Group - genetics; Diuron - pharmacology; DNA, Fungal - genetics; DNA, Mitochondrial - genetics; Drug Resistance, Microbial; Fundamental and applied biological sciences. Psychology; Genetics of eukaryotes. Biological and molecular evolution; Mutation; Protein Conformation; Saccharomyces cerevisiae; Saccharomyces cerevisiae - genetics; Structure-Activity Relationship; Thallophyta, bryophyta; Vegetals; (Saccharomyces cerevisiae) Diuron resistance Mitochondrial cytochrome b split gene DNA sequence analysis Primer extension Mutational alteration; Yeast; RNA; Nucleotide sequence; Reverse transcription; Diuron; Fungi; Respiratory chain; Sensitivity resistance; Primer template system; Ascomycetes; Mutation; Saccharomyces cerevisiae; Thallophyta
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(86)81019-5

Diuron (3-[34-dichlorophenyl]-1,1-dimethylurea), an inhibitor of mitochondrial respiration blocks the yeast respiratory chain between cytochrome b and c 1. Diuron-resistant mutants of Saccharomyces cerevisiae have been selected and several mutations localized to the mitochondrial cytochrome b gene. The present paper identifies specific DNA base changes within the cytochrome b gene conferring diuron-resistance. DNA sequence analysis was done utilizing primer extension of crude mitochondrial RNA preparations in the presence of reverse transcriptase. Five independent diuron-resistant mutations have been sequenced.