滇池沉积物中好氧氨氧化细菌群落多样性研究

从滇池沉积物中提取好氧氨氧化细菌（aerobic ammonium-oxidizing bacteria,AOB）的DNA,通过构建硝化作用氨单加氧酶基因（amo A）文库并划分操作分类单元（OTU）的方法,揭示好氧氨氧化细菌群落结构多样性。获得了80个amo A基因克隆,利用Mothur软件将相似度不小于97%的序列归为1个OTU,共得到22个OTUs,其中OTU1、OTU2、OTU3单元最大,分别包含了27、12、9条序列。对22个OTUs的代表序列进行系统发育分析表明,其序列都属于变形杆菌的β-亚纲,分布于亚硝化螺菌属、亚硝化弧菌属、亚硝化单胞菌属、亚硝化叶菌属4个属;优势种群为Nitr...

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Published in西南农业学报 Vol. 29; no. 3; pp. 689 - 694
Main Author 陈泽斌 李冰 夏体渊 王定康 余磊 任禛 靳松 何峰
Format Journal Article
LanguageChinese
Published 云南省都市特色农业工程技术研究中心,云南昆明650214%中国科学院昆明动物研究所,云南昆明,650223%昆明学院农学院,云南昆明,650214%昆明学院生命科学与技术系,云南昆明,650214%昆明市滇池生态研究所,云南昆明,650228 2016
昆明学院农学院,云南昆明650214
Subjects
好氧氨氧化菌
氨单加氧酶基因
沉积物
滇池
Aerobic ammonium-oxidizing bacteria
Sediment
amoA
滇池
沉积物
氨单加氧酶基因
好氧氨氧化菌
Dianchi lake
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ISSN1001-4829
DOI10.16213/j.cnki.scjas.2016.03.042

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Summary:从滇池沉积物中提取好氧氨氧化细菌（aerobic ammonium-oxidizing bacteria,AOB）的DNA,通过构建硝化作用氨单加氧酶基因（amo A）文库并划分操作分类单元（OTU）的方法,揭示好氧氨氧化细菌群落结构多样性。获得了80个amo A基因克隆,利用Mothur软件将相似度不小于97%的序列归为1个OTU,共得到22个OTUs,其中OTU1、OTU2、OTU3单元最大,分别包含了27、12、9条序列。对22个OTUs的代表序列进行系统发育分析表明,其序列都属于变形杆菌的β-亚纲,分布于亚硝化螺菌属、亚硝化弧菌属、亚硝化单胞菌属、亚硝化叶菌属4个属;优势种群为Nitrosomonas europaea的近缘种,尚未被描述过。
Bibliography:CHEN Ze-hin, LI Bing , XIA Ti-yuan , WANG Ding-kang, YU Lei , PEN Zhen ,TIN Song, HE Feng （ 1. Agriculture College, Kunming University, Yunnan Kunming 650214, China; 2. Engineering Research Center for Characteristics Agrlculture of Yunnan Province, Yunnan Kunming 650214, China; 3. Kunming Institute of Zoology, Chinese Academy of Sciences, Yunnan Kunming 650223, China; 4. Life Science and Technology Department, Kunming University, Yunnan Kunming 650214, China; 5. Kunming Institute of Ecology of Dianchi Lake, Yunnan Kunming 650228, China）
The clone libraries of ammonium monooxygenase encoding gene （amoA） in the sediments of Dianchi lake were constructed and grouped into the operational taxonomic units （OTUs） to determine the community diversity of aerobic ammonium-oxidizing bacteria （AOB）. A total of 80 sequences were obtained from Dianchi lake. 22 OTUs from Dianchi lake were grouped respectively based on a 3 % amoA gene sequence distance cutoff with the Mothur program. The largest groups were OTU1, OTU2 and OTU
ISSN:1001-4829
DOI:10.16213/j.cnki.scjas.2016.03.042