香港牡蛎精子超低温冷冻保存的研究
采用程序降温法对香港牡蛎（Crassostrea hongkongensis）精子进行超低温冷冻保存,利用两种抗冻保护液筛选最佳降温程序、稀释比、平衡时间和人工授精精卵比,建立了一套成熟的香港牡蛎精子超低温冷冻保存技术.结果表明,降温程序Ⅳ、精液/抗冻保护液稀释比为1∶40及平衡时间为30min时复苏精子的存活率和活力最高,而且以生理盐水为稀释液的抗冻保护液A的精子存活率明显高于以柠檬酸钠-甘氨酸为稀释液的抗冻保护液B,冻精人工授精精卵比为40∶1时受精率与鲜精水平接近（达83％）.研究成果为香港牡蛎优良种质的长期保存提供了科学依据和技术支持....
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| Published in | 西南农业学报 Vol. 27; no. 5; pp. 2229 - 2235 |
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| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
广西遗传育种与健康养殖重点实验室,广西壮族自治区水产科学研究院,广西南宁530021
2014
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1001-4829 |
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| Summary: | 采用程序降温法对香港牡蛎（Crassostrea hongkongensis）精子进行超低温冷冻保存,利用两种抗冻保护液筛选最佳降温程序、稀释比、平衡时间和人工授精精卵比,建立了一套成熟的香港牡蛎精子超低温冷冻保存技术.结果表明,降温程序Ⅳ、精液/抗冻保护液稀释比为1∶40及平衡时间为30min时复苏精子的存活率和活力最高,而且以生理盐水为稀释液的抗冻保护液A的精子存活率明显高于以柠檬酸钠-甘氨酸为稀释液的抗冻保护液B,冻精人工授精精卵比为40∶1时受精率与鲜精水平接近（达83％）.研究成果为香港牡蛎优良种质的长期保存提供了科学依据和技术支持. |
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| Bibliography: | 51-1213/S The cryopreservation of Crassostrea hongkongensis sperm were preserved by programmed cooling method,two cryoprotective solution were used to screen different,the different ratio of dilution,the different balance time and different ratio of sperm and ovum in artificial insemination,In the research that we established a technology of spermatozoa cryopreservation.The result discovered that the survival ratio and sperm motility were high in the cryopreservation Ⅳ program,the ratio of sperm/cryoprotective solution was 1 ∶ 40 and the balance time was 30 min.In the ratio of sperm and ovum by artificial insemination,we found that the ration of fertilization and the level of fresh sperms was 83 ％.The research provided of science basis and technology support for long-term savepreservekeep of excellent germplasm Crassostrea hongkongensis. Crassostrea hongkongensis; Sperm cryopreservation; Programmed cooling method; The ratio of dilution; Artificial insemination ZHAO Yong-zhen,CHEN Xiu-li,YANG Chun-ling,PENG Min,L |
| ISSN: | 1001-4829 |