石蒜碱诱导肺癌NCI-H460细胞凋亡作用及其机制

目的通过研究石蒜碱在体外对人肺癌NCI-H460细胞增殖抑制和诱导凋亡的作用,探讨石蒜碱抗肿瘤机制。方法 MTT法检测石蒜碱对NCI-H460细胞增殖的抑制作用,流式细胞术检测NCI-H460细胞的凋亡率,比色法检测凋亡因子相关因子Caspase 3活性。结果石蒜碱能够有效抑制肺癌NCI-H460细胞增殖,IC_（50）为（5.79±0.11）μmol/L,对NCI-H460细胞的凋亡具有诱导效应,可增强肺癌Caspase 3的活性。结论石蒜碱在体外能有效地抑制NCI-H460细胞增殖并促进其凋亡,其机制可能与激活Caspase 3的表达从而诱导肿瘤细胞凋亡有关。...

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Bibliographic Details
Published in肿瘤防治研究 Vol. 45; no. 1; pp. 5 - 8
Main Author 李居伟;杨扬
Format Journal Article
LanguageChinese
Published 广西肝脏损伤与修复分子医学重点实验室 541004 桂林%广西肝脏损伤与修复分子医学重点实验室 541004 桂林 2018
桂林医学院临床医学院 541004 桂林
桂林医学院药学院 541004 桂林
Subjects
3
Caspase
NCI-H460细胞
石蒜碱
细胞凋亡
肺癌
细胞凋亡
NCI-H460 cells
肺癌
Lung cancer
Caspase 3
石蒜碱
Lycorine
NCI-H460细胞
Apoptosis
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Summary:目的通过研究石蒜碱在体外对人肺癌NCI-H460细胞增殖抑制和诱导凋亡的作用,探讨石蒜碱抗肿瘤机制。方法 MTT法检测石蒜碱对NCI-H460细胞增殖的抑制作用,流式细胞术检测NCI-H460细胞的凋亡率,比色法检测凋亡因子相关因子Caspase 3活性。结果石蒜碱能够有效抑制肺癌NCI-H460细胞增殖,IC_（50）为（5.79±0.11）μmol/L,对NCI-H460细胞的凋亡具有诱导效应,可增强肺癌Caspase 3的活性。结论石蒜碱在体外能有效地抑制NCI-H460细胞增殖并促进其凋亡,其机制可能与激活Caspase 3的表达从而诱导肿瘤细胞凋亡有关。
Bibliography:42-1241/R
Objective To investigate the effect of lycorine on the proliferation inhibition and apoptosis induction in human lung cancer NCI-H460 cells, and to explore the anti-tumor mechanism of lycorine. Methads MTT assay was used to detect the proliferation of NCI-H460 cells. The apoptosis rate of NCI-H460 cells was detected by flow cytometry. The activity of Caspase 3 was detected by colorimetry. Results Lycorine could inhibit the proliferation of NCI-H460 cells, and the IC50 was （5.79±0.11）μmol/L. Lycorine had an induced effect on the apoptosis of NCI-H460 cells. Lycorine could enhance Caspase 3 activity. Canclusion Lycorine could effectively inhibit the proliferation and induce the apoptosis of NCI-H460 cells in vitro, and its mechanism may be related to the activation of Caspase 3 expression and the apoptosis induce of tumor cells.
LI Juwei1,2, YANG Yang2,3 （1. College of Clinical Medicine, Guilin Medical University, Guilin 541004, China; 2. Guangxi Key Laboratory of Molecular Medicine in Liver Injury and
ISSN:1000-8578
DOI:10.3971/j.issn.1000-8578.2018.17.0388