Toxins and virulence factors of enterotoxigenic Escherichia coli associated with strains isolated from indigenous children and international visitors to a rural community in Guatemala

• Published in: Epidemiology and infection Vol. 143; no. 8; pp. 1662 - 1671
• Main Authors: TORRES, O. R., GONZÁLEZ, W., LEMUS, O., PRATDESABA, R. A., MATUTE, J. A., WIKLUND, G., SACK, D. A., BOURGEOIS, A. L., SVENNERHOLM, A-M.
• Format: Journal Article
• Language: English
• Published: Cambridge, UK Cambridge University Press 01.06.2015
• Subjects: Adult; Bacterial Toxins - metabolism; Child, Preschool; Children & youth; COLONIZATION FACTORS; DEVELOPING-COUNTRIES; Diarrhea; Diarrhea - epidemiology; Diarrhea - microbiology; Diarrhoea; DISEASE BURDEN; DOUBLE-BLIND; E coli; enteric bacteria; Enterotoxigenic Escherichia coli - genetics; Enterotoxigenic Escherichia coli - metabolism; enterotoxin; Enterotoxins - metabolism; Environmental & Occupational Health; Escherichia coli; Escherichia coli Infections - epidemiology; Escherichia coli Infections - microbiology; Escherichia coli Proteins - metabolism; ETEC; FIELD; Gastroenterologi; Gastroenterology and Hepatology; Gastrointestinal Infections; Guatemala; Humans; Illnesses; Infant; Infectious Diseases; KILLED ORAL VACCINE; Laboratories; LDCs; Morbidity; Mortality; Original Papers; Pathogens; Population Groups; PREVALENCE; Public; Quality control; Rural Population; Travel; TRAVELERS DIARRHEA; travellers' infection; TRIAL; Vaccines; Virulence Factors - metabolism; YOUNG-CHILDREN; Guatemala; Bangladesh; United States > US; Antigua; enterotoxin; Diarrhoea; travellers' infection; Escherichia coli; enteric bacteria
• ISSN: 0950-2688; 1469-4409
• DOI: 10.1017/S0950268814002295

Diarrhoea remains a common cause of illness in Guatemala, with children suffering most frequently from the disease. This study directly compared the frequency, enterotoxin, and colonization factor (CF) profiles of enterotoxigenic Escherichia coli (ETEC) strains isolated from children living in a rural community in Guatemala and from Western visitors to the same location during the same seasons, using similar detection methodologies. We found that ETEC accounted for 26% of severe cases of diarrhoea in children requiring hospitalization, 15% of diarrhoea in the community, and 29% of travellers' diarrhoea in visitors staying ⩾2 weeks. The toxin and CF patterns of the ETEC strains isolated from both groups differed significantly (P < 0·0005) as determined by χ 2 = 60·39 for CFs and χ 2 = 35 for toxins, while ETEC phenotypes found in Guatemalan children were comparable to those found in children from other areas of the world.