Large number of rebounding/founder HIV variants emerge from multifocal infection in lymphatic tissues after treatment interruption

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 112; no. 10; pp. E1126 - E1134
• Main Authors: Rothenberger, Meghan K., Keele, Brandon F., Wietgrefe, Stephen W., Fletcher, Courtney V., Beilman, Gregory J., Chipman, Jeffrey G., Khoruts, Alexander, Estes, Jacob D., Anderson, Jodi, Callisto, Samuel P., Schmidt, Thomas E., Thorkelson, Ann, Reilly, Cavan, Perkey, Katherine, Reimann, Thomas G., Utay, Netanya S., Makamdop, Krystelle Nganou, Stevenson, Mario, Douek, Daniel C., Haase, Ashley T., Schacker, Timothy W.
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 10.03.2015; National Acad Sciences
• Series: PNAS Plus
• Subjects: Adult; Anti-HIV Agents - administration & dosage; Anti-HIV Agents - therapeutic use; Antiretroviral drugs; Biological Sciences; CD4 Lymphocyte Count; Cells; Drug Administration Schedule; Drug therapy; HIV; HIV - genetics; HIV - physiology; HIV Infections - drug therapy; HIV Infections - virology; Human immunodeficiency virus; Humans; In Situ Hybridization; Lymphatic system; Lymphoid Tissue - virology; Middle Aged; Molecular Sequence Data; Phylogeny; PNAS Plus; RNA, Viral - blood; Tissues; Viral Load; HIV/AIDS; treatment interruption; viral recrudescence; founder population
• ISSN: 0027-8424; 1091-6490; 1091-6490
• DOI: 10.1073/pnas.1414926112

Significance Antiretroviral therapy (ART) effectively suppresses HIV replication; however, treatment cannot be stopped, because latently infected CD4+ T cells will rekindle infection. As one estimate of the size of the pool of latently infected cells that must be purged for cure, we asked whether recrudescent infection is the result of reactivation from one or a larger number latently infected cells. We briefly stopped ART in fully suppressed patients to see how widespread new infections were in the lymphoid tissues (LTs) and how diverse rebounding/founder viruses were in peripheral blood. Recrudescent infection was detectable in multiple different LTs, and the population was genetically diverse, consistent with reactivation from a larger number of cells. These findings underscore the challenges facing strategies to eradicate HIV infection. Antiretroviral therapy (ART) suppresses HIV replication in most individuals but cannot eradicate latently infected cells established before ART was initiated. Thus, infection rebounds when treatment is interrupted by reactivation of virus production from this reservoir. Currently, one or a few latently infected resting memory CD4 T cells are thought be the principal source of recrudescent infection, but this estimate is based on peripheral blood rather than lymphoid tissues (LTs), the principal sites of virus production and persistence before initiating ART. We, therefore, examined lymph node (LN) and gut-associated lymphoid tissue (GALT) biopsies from fully suppressed subjects, interrupted therapy, monitored plasma viral load (pVL), and repeated biopsies on 12 individuals as soon as pVL became detectable. Isolated HIV RNA-positive (vRNA+) cells were detected by in situ hybridization in LTs obtained before interruption in several patients. After interruption, multiple foci of vRNA+ cells were detected in 6 of 12 individuals as soon as pVL was measureable and in some subjects, in more than one anatomic site. Minimal estimates of the number of rebounding/founder (R/F) variants were determined by single-gene amplification and sequencing of viral RNA or DNA from peripheral blood mononuclear cells and plasma obtained at or just before viral recrudescence. Sequence analysis revealed a large number of R/F viruses representing recrudescent viremia from multiple sources. Together, these findings are consistent with the origins of recrudescent infection by reactivation from many latently infected cells at multiple sites. The inferred large pool of cells and sites to rekindle recrudescent infection highlights the challenges in eradicating HIV.