Cloning of the Human Homolog of the CDC34 Cell Cycle Gene by Complementation in Yeast

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 90; no. 22; pp. 10484 - 10488
• Main Authors: Plon, Sharon E., Leppig, Kathleen A., Do, Hong-Nhung, Groudine, Mark
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences of the United States of America 15.11.1993; National Acad Sciences; National Academy of Sciences
• Subjects: amino acid sequence; Anaphase-Promoting Complex-Cyclosome; Base Sequence; CDC34 gene; cDNA; Cell Cycle; Cell lines; chromosome 19; Chromosome Mapping; Chromosomes, Human, Pair 19; Cloning, Molecular; Complementary DNA; Deoxyribonucleic acid; DNA; DNA Primers; Genes; Genetic Complementation Test; Genetic mutation; Genetics; Human chromosomes; Humans; In Situ Hybridization, Fluorescence; Ligases - genetics; man; Molecular Sequence Data; nucleotide sequence; Plasmids; prediction; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae Proteins; Sequence Alignment; Sequence Homology, Amino Acid; Somatic cells; Ubiquitin-Conjugating Enzymes; Ubiquitin-Protein Ligase Complexes; Ubiquitin-Protein Ligases; Ubiquitins - metabolism; Yeasts
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.90.22.10484

In a screen designed to isolate human cDNAs that complement a yeast G2phase checkpoint mutation (mec1), we isolated a cDNA homologous to the Saccharomyces cerevisiae CDC34 gene. The human CDC34 cDNA can functionally substitute for the yeast CDC34 gene and represents a mammalian homolog of the group of yeast genes required for the late G1→ S phase transition. The human CDC34 gene is expressed in multiple cell lines as a unique species and Southern blot analysis reveals evidence for a single gene that is highly conserved in higher eukaryotes. The human gene is located on the far telomeric region of 19p13.3 in a location that defines a region of homology between human chromosome 19p and mouse chromosome 11.