Genetic analysis of cysteine-poor prolamin polypeptides reduced in the endosperm of the rice esp1 mutant

• Published in: Plant science (Limerick) Vol. 181; no. 2; pp. 125 - 131
• Main Authors: Ushijima, Tomokazu, Matsusaka, Hiroaki, Jikuya, Hiroyuki, Ogawa, Masahiro, Satoh, Hikaru, Kumamaru, Toshihiro
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 01.08.2011; [Ireland]: Elsevier Science Ireland Ltd; Elsevier
• Subjects: Biological and medical sciences; Chromosomes, Plant - genetics; Cysteine - metabolism; Electrophoresis, Gel, Two-Dimensional; Electrophoresis, Polyacrylamide Gel; endosperm; Endosperm - chemistry; Endosperm - genetics; Endosperm - metabolism; Fundamental and applied biological sciences. Psychology; Isoelectric Focusing; loci; MALDI-TOF MS; matrix-assisted laser desorption-ionization mass spectrometry; Multigene Family; Mutant; mutants; Mutation; Oryza - chemistry; Oryza - genetics; Oryza - metabolism; Oryza sativa; Plant Proteins - genetics; Plant Proteins - metabolism; polypeptides; Prolamin; prolamins; Prolamins - chemistry; Prolamins - genetics; Prolamins - metabolism; Rice; RNA, Plant - genetics; Seeds - chemistry; Seeds - genetics; Seeds - metabolism; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods; Starchy endosperm; Storage protein; structural genes; substitution lines; MALDI-TOF MS; Starchy endosperm; Storage protein; Prolamin; Mutant; Rice; Monocotyledones; Cysteine; Time of flight spectrometer; Plant biology; Matrix assisted laser desorption ionization; Genetic determinism; Oryza; Gramineae; Polypeptide; Angiospermae; Herbaceous plant; Spermatophyta; Endosperm; Mutation; Mass spectrometry
• ISSN: 0168-9452; 1873-2259
• DOI: 10.1016/j.plantsci.2011.04.011

The esp1 mutant CM21 specifically exhibits reduced levels of cysteine-poor (CysP) prolamin bands with pIs of 6.65, 6.95, 7.10, and 7.35 in rice seed. Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis demonstrated that the bands with pIs 6.65, 6.95, and 7.35 are encoded by different structural genes. These results suggest that the Esp1 locus encodes a regulatory factor involved in the synthesis and/or accumulation of CysP prolamin molecules. Isoelectric focusing (IEF) analysis of CysP prolamins in chromosome substitution lines showed that structural genes for bands with pI values of 6.95, 7.10, and 7.35, which are reduced in esp1 mutant lines, are located as a gene cluster in the 44.2cM region on chromosome 5.