Chemical composition of lipopolysaccharides isolated from various endophytic nitrogen-fixing bacteria of the genus Herbaspirillum

• Published in: Canadian journal of microbiology Vol. 56; no. 4; pp. 342 - 347
• Main Authors: Serrato, R.V, Sassaki, G.L, Cruz, L.M, Carlson, R.W, Muszyński, A, Monteiro, R.A, Pedrosa, F.O, Souza, E.M, Iacomini, M
• Format: Journal Article
• Language: English
• Published: Canada NRC Research Press 01.04.2010; Canadian Science Publishing NRC Research Press
• Subjects: Bacteria; Bacteriology; bactérie diazotrophe; carbohydrate composition; Carbohydrates; cell membranes; diazotrophic bacteria; endophytes; Fixation; Flowers & plants; Herbaspirillum; Herbaspirillum - chemistry; Herbaspirillum - physiology; Herbaspirillum seropedicae; lipopolysaccharide; Lipopolysaccharides; Lipopolysaccharides - chemistry; LPS; Mitogens; Nitrogen; Nitrogen Fixation; nitrogen-fixing bacteria; Oryza sativa; Plant growth; Poaceae - microbiology; Sorghum; Triticum aestivum; Zea mays; United States
• ISSN: 0008-4166; 1480-3275
• DOI: 10.1139/W10-011

Bacteria from the genus Herbaspirillum are endophytes responsible for nitrogen fixation in gramineous plants of economic importance such as maize, sugarcane, sorghum, rice, and wheat. Some species are known to produce plant growth substances. In contrast, Herbaspirillum rubrisubalbicans strains are known to be mild plant pathogens. The molecular communication between the plant and the microbes might involve lipopolysaccharides present in the outer membrane of these gram-negative bacteria. Phenol-water extraction was used to obtain lipopolysaccharides from 7 strains of Herbaspirillum seropedicae (SmR1, Z67, Z78, ZA95, and M2) and H. rubrisubalbicans (M1 and M4). The electrophoretic profiles and chemical composition of the lipopolysaccharides obtained in the phenol and aqueous extracts were shown herein.