Transcriptome Analysis Reveals a Major Gene Expression Pattern and Important Metabolic Pathways in the Control of Heterosis in Chinese Cabbage

Although heterosis is commonly used in Chinese cabbage, its molecular basis is poorly understood. In this study, 16Chinese cabbage hybrids were utilized as test subjects to explore the potential molecular mechanism of heterosis. RNA sequencing revealed 5815–10,252 differentially expressed genes (DEG...

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Bibliographic Details
Published inPlants (Basel) Vol. 12; no. 5; p. 1195
Main Authors Li, Ru, Nie, Shanshan, Zhang, Ning, Tian, Min, Zhang, Lugang
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 06.03.2023
MDPI
Subjects
Agricultural production
Analysis
Biosynthesis
Brassica oleracea
cabbage
Chinese cabbage
Circadian rhythm
Circadian rhythms
Corn
differentially expressed genes
Females
Gene expression
gene expression regulation
Gene sequencing
Genes
Genetic aspects
Genetic research
Heterosis
host-pathogen relationships
Hybrids
Hypotheses
major genes
Males
metabolic pathway
Metabolic pathways
Metabolism
Metabolites
MicroRNAs
Pathogens
Photosynthesis
Physiological aspects
Proteins
RNA
RNA sequencing
transcriptome analysis
Transcriptomes
transcriptomics
China
heterosis
metabolic pathway
differentially expressed genes
transcriptome analysis
Chinese cabbage
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Summary:Although heterosis is commonly used in Chinese cabbage, its molecular basis is poorly understood. In this study, 16Chinese cabbage hybrids were utilized as test subjects to explore the potential molecular mechanism of heterosis. RNA sequencing revealed 5815–10,252 differentially expressed genes (DEGs) (female parent vs. male parent), 1796–5990 DEGs (female parent-vs-hybrid), and 2244–7063 DEGs (male parent vs. hybrid) in 16 cross combinations at the middle stage of heading. Among of them, 72.83–84.20% DEGs conformed to the dominant expression pattern, which is the predominant expression pattern in hybrids. There were 13 pathways in which DEGs were significantly enriched in most cross combinations. Among them, the plant–pathogen interaction (ko04626) and circadian rhythm-plant (ko04712)were significantly enriched by DEGs in strong heterosis hybrids. WGCNA also proved that the two pathways were significantly related to heterosis in Chinese cabbage.
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ISSN:2223-7747
2223-7747
DOI:10.3390/plants12051195