Lawsonia inermis-mediated synthesis of silver nanoparticles: activity against human pathogenic fungi and bacteria with special reference to formulation of an antimicrobial nanogel

• Published in: IET nanobiotechnology Vol. 8; no. 3; pp. 172 - 178
• Main Authors: Gupta, Arpita, Bonde, Shital R, Gaikwad, Swapnil, Ingle, Avinash, Gade, Aniket K, Rai, Mahendra
• Format: Journal Article
• Language: English
• Published: Stevenage The Institution of Engineering and Technology 01.09.2014; IET; The Institution of Engineering & Technology
• Subjects: Anti-Infective Agents - chemical synthesis; Anti-Infective Agents - pharmacology; antibacterial activity; antibiotics; Antiinfectives and antibacterials; antimicrobial activity; antimicrobial nanogel formulation; antimicrobial nanoparticle gel; Bacteria; Bacterial Physiological Phenomena - drug effects; bactericidal agents; biochemistry; biodiffusion; Biological and medical sciences; biomedical materials; bioreduction; biosynthesis route; Biotechnology; C. albicans; Candida albicans; capped nanoparticle formation; Cell Survival - drug effects; Cell Survival - physiology; cellular biophysics; chemical agents; disc diffusion method; Drug Compounding - methods; drugs; drug‐resistant microorganisms; Fourier transform infrared spectroscopy; Fourier transform spectra; Fourier transforms; Fundamental and applied biological sciences. Psychology; Fungi - drug effects; Fungi - physiology; fungicidal agents; gels; human pathogenic fungi; Humans; infrared spectra; Infrared spectroscopy; intermediate complex formation; L. inermis; Lawsonia; Lawsonia inermis mediated synthesis; leaf extract; M. canis; Materials Testing; microbial resistance; microorganisms; Microsporum canis; nanofabrication; nanomedicine; nanoparticle tracking; Nanoparticles; Nanoparticles - administration & dosage; Nanoparticles - chemistry; Nanostructure; Nanostructures - chemistry; Nanostructures - ultrastructure; P. acne; Particle Size; Plant Extracts - chemistry; Plant Leaves - chemistry; Propioniabacterium acne; reduction (chemical); Silver; Silver - chemistry; Silver - pharmacology; silver nanoparticles; Synthesis; T. mentagrophytes; transmission electron microscopy; Trichophyton mentagrophytes; ultraviolet spectra; UV‐vis spectrophotometer; UV‐visible spectrophotometer; visible spectra; zeta potential; antibacterial activity; cellular biophysics; biodiffusion; visible spectra; nanoparticles; transmission electron microscopy; drug-resistant microorganisms; leaf extract; disc diffusion method; Microsporum canis; nanoparticle tracking; fungicidal agents; Propioniabacterium acne; capped nanoparticle formation; bactericidal agents; C. albicans; biomedical materials; infrared spectra; Fourier transform spectra; silver; Fourier transform infrared spectroscopy; P. acne; antibiotics; intermediate complex formation; Candida albicans; Ag; nanofabrication; biosynthesis route; Trichophyton mentagrophytes; M. canis; chemical agents; gels; human pathogenic fungi; reduction (chemical); bioreduction; L. inermis; antimicrobial nanoparticle gel; drugs; nanomedicine; zeta potential; antimicrobial activity; UV-visible spectrophotometer; UV-vis spectrophotometer; ultraviolet spectra; silver nanoparticles; microorganisms; T. mentagrophytes; biochemistry; microbial resistance; antimicrobial nanogel formulation; Lawsonia inermis mediated synthesis; Nanobiotechnology
• ISSN: 1751-8741; 1751-875X; 1751-875X
• DOI: 10.1049/iet-nbt.2013.0015

Lawsonia inermis mediated synthesis of silver nanoparticles (Ag-NPs) and its efficacy against Candida albicans, Microsporum canis, Propioniabacterium acne and Trichophyton mentagrophytes is reported. A two-step mechanism has been proposed for bioreduction and formation of an intermediate complex leading to the synthesis of capped nanoparticles was developed. In addition, antimicrobial gel for M. canis and T. mentagrophytes was also formulated. Ag-NPs were synthesized by challenging the leaft extract of L. inermis with 1 mM AgNO3. The Ag-NPs were characterized by Ultraviolet-Visible (UV–Vis) spectrophotometer and Fourier transform infrared spectroscopy (FTIR). Transmission electron microscopy (TEM), nanoparticle tracking and analysis sytem (NTA) and zeta potential was measured to detect the size of Ag-NPs. The antimicrobial activity of Ag-NPs was evaluated by disc diffusion method against the test organisms. Thus these Ag-NPs may prove as a better candidate drug due to their biogenic nature. Moreover, Ag-NPs may be an answer to the drug-resistant microorganisms.