Nucleoside diphosphate kinase III is localized to the inter-membrane space in plant mitochondria

• Published in: FEBS letters Vol. 508; no. 2; pp. 272 - 276
• Main Authors: Sweetlove, L.J, Mowday, B, Hebestreit, H.F, Leaver, C.J, Millar, A.H
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 16.11.2001
• Subjects: Amino Acid Sequence; Arabidopsis - cytology; Arabidopsis - enzymology; Electrophoresis, Gel, Two-Dimensional; IEF, isoelectric focusing; IMS, inter-membrane space; Mass Spectrometry; Mitochondria - enzymology; Molecular Sequence Data; Molecular Weight; NDPK, nucleoside diphosphate kinase; Nucleoside diphosphate kinase; Nucleoside-Diphosphate Kinase - analysis; Nucleoside-Diphosphate Kinase - chemistry; Nucleoside-Diphosphate Kinase - metabolism; Peptide Mapping; Phylogeny; Plant mitochondrion; Proteomic; Sequence Homology, Amino Acid; Solanum tuberosum - cytology; Solanum tuberosum - enzymology; IMS, inter-membrane space; Plant mitochondrion; IEF, isoelectric focusing; NDPK, nucleoside diphosphate kinase; Nucleoside diphosphate kinase; Proteomic
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/S0014-5793(01)03069-1

Three types of nucleoside diphosphate kinases (NDPKs) are found in plants but the intra-cellular compartmentation of these proteins is not certain, especially the location of the recently identified type III proteins. Through the fractionation of plant mitochondria from potato and Arabidopsis, display of protein profiles by 2D gel electrophoresis, and identification by mass spectrometry, we present the first direct evidence that type III proteins are localized in the inter-membrane space of plant mitochondria. The possible metabolic functions of NDPK III are discussed in light of its sub-cellular localization.