辣椒种间杂交花粉母细胞减数分裂行为研究

研究辣椒种间杂交花粉母细胞减数分裂行为,有助于育种工作者了解辣椒的微观发育行为并掌握辣椒种间杂交不育的具体原因。通过改良的染色体制片方法共观察了从前期I至四分孢子时期的486个花粉母细胞,发现辣椒花粉母细胞减数分裂行为中细胞质分裂类型为同时型,且染色体有多种异常行为。中期Ⅱ的细胞中有23.8%出现染色体滞后现象,另外,在前期Ⅰ、中期Ⅰ、后期Ⅰ中也存在少量的染色体异常行为。末期Ⅱ中产生微核的概率为7.0%。这一结果可以较好地解释辣椒种间杂交花粉育性和结实率低的现象,为进一步创制新的种质提供一定的理论依据。...

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Bibliographic Details
Published in江西农业学报 Vol. 27; no. 6; pp. 1 - 6
Main Author 刘娜 刁卫平 郭广君 潘宝贵 戈伟 许亚池 王述彬 刘金兵
Format Journal Article
LanguageChinese
Published 江苏省农业科学院 蔬菜研究所，江苏 南京 210014 2015
南京农业大学 园艺学院，江苏 南京 210095%江苏省农业科学院 蔬菜研究所,江苏 南京,210014
Subjects
减数分裂
种间杂交
花粉母细胞
辣椒
花粉母细胞
种间杂交
Pepper
Interspecific hybridization
Pollen mother cell
减数分裂
Meiosis
辣椒
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ISSN1001-8581

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Summary:研究辣椒种间杂交花粉母细胞减数分裂行为,有助于育种工作者了解辣椒的微观发育行为并掌握辣椒种间杂交不育的具体原因。通过改良的染色体制片方法共观察了从前期I至四分孢子时期的486个花粉母细胞,发现辣椒花粉母细胞减数分裂行为中细胞质分裂类型为同时型,且染色体有多种异常行为。中期Ⅱ的细胞中有23.8%出现染色体滞后现象,另外,在前期Ⅰ、中期Ⅰ、后期Ⅰ中也存在少量的染色体异常行为。末期Ⅱ中产生微核的概率为7.0%。这一结果可以较好地解释辣椒种间杂交花粉育性和结实率低的现象,为进一步创制新的种质提供一定的理论依据。
Bibliography:LIU Na, DIAO Wei-ping, GUO Guang-jun, PAN Bao-gui, GE Wei, XU Ya-chi, WANG Shu-bin, LIU Jin-bing （ 1. Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; 2. College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China）
Pepper;Interspecific hybridization;Pollen mother cell;Meiosis
36-1124/S
The research on the meiosis behavior of pollen mother cells in pepper interspecific hybrids can help breeders to understand the microcosmic developmental behavior of pepper and to grasp the definite reasons for pepper interspecific hybrid sterility. In this experiment, the improved chromosome microslide-preparing method was used to observe 486 pepper pollen mother cells from prophaseⅠto quartet stage.It was found that the cytokinesis of pepper pollen mother cells in meiosis behavior was of simultaneous type, and the chromosomes had multiple abnormal behaviors.Chromosome hysteresis occurred in 23.8%of metaphase-Ⅱ cells.In addition, a small quantity of chromosomal abn
ISSN:1001-8581