经卡介苗活化的树突状细胞疫苗治疗胰腺癌的基础及临床运用展望——暨向2011年诺贝尔医学奖得主Ralph Steinman教授致敬

目的探讨经卡介苗（BCG）活化的树突状细胞（DC）疫苗体外直接抗胰腺癌细胞Panc02的机制,以及观察经不同部位注射DC疫苗抗小鼠胰腺癌移植瘤的效应。方法从C57BL/6小鼠骨髓中诱导培养DC,并予以BCG促成熟,用流式细胞技术检测DC成熟度,CCK-8细胞计数检测DC直接抑制Panc02增殖情况,流式细胞仪检测BCG促成熟DC表面凋亡诱导配体（TRAIL）的表达量。C57BL/6小鼠皮下接种Panc02胰腺癌细胞制成荷瘤小鼠,第7 d予以DC疫苗注射免疫治疗（DC疫苗为经Panc02细胞冻融抗原致敏后BCG促成熟的DC）,分为3组：瘤内注射生理盐水组、皮下注射DC疫苗组和瘤内注射DC疫苗组,...

Full description

Saved in:
Bibliographic Details
Published in临床肝胆病杂志 Vol. 28; no. 8; pp. 592 - 597
Main Author 刘文佳 朱浩 吕瑛 李振磊 张松 裴庆山 邹晓平
Format Journal Article
LanguageChinese
Published 南京大学医学院附属鼓楼医院消化科,南京,210008 2012
Subjects
TNF相关凋亡诱导配体
卡介苗
树突细胞
胰腺肿瘤
树突细胞
胰腺肿瘤
卡介苗
TNF相关凋亡诱导配体
Online AccessGet full text

Cover

More Information
Summary:目的探讨经卡介苗（BCG）活化的树突状细胞（DC）疫苗体外直接抗胰腺癌细胞Panc02的机制,以及观察经不同部位注射DC疫苗抗小鼠胰腺癌移植瘤的效应。方法从C57BL/6小鼠骨髓中诱导培养DC,并予以BCG促成熟,用流式细胞技术检测DC成熟度,CCK-8细胞计数检测DC直接抑制Panc02增殖情况,流式细胞仪检测BCG促成熟DC表面凋亡诱导配体（TRAIL）的表达量。C57BL/6小鼠皮下接种Panc02胰腺癌细胞制成荷瘤小鼠,第7 d予以DC疫苗注射免疫治疗（DC疫苗为经Panc02细胞冻融抗原致敏后BCG促成熟的DC）,分为3组：瘤内注射生理盐水组、皮下注射DC疫苗组和瘤内注射DC疫苗组,1周后再治疗1次,第2次治疗后15 d处死小鼠,观察不同治疗抗肿瘤的效果。结果 BCG活化后DC疫苗成熟度明显增加,其表型CD86表达增加;经BCG活化成熟的DC能直接抑制Panc02细胞生长,这一作用可被TRAIL抗体部分抑制,且流式细胞术检测发现成熟DC表面分子TRAIL明显高于未经BCG促成熟组未成熟DC。肿瘤体重：瘤内注射DC疫苗组〈皮下注射DC疫苗组〈瘤内注射生理盐水组（P〈0.01）。结论 BCG活化的胰腺癌DC疫苗的生物活性明显增加,并可通过TRAIL这一途径直接杀伤肿瘤细胞;且经BCG活化的DC疫苗瘤内注射免疫治疗抗肿瘤作用更强。
Bibliography:LIU Wen -fia, ZHU Hao, LYU Ying, et al. （ Department of Gastroenterology, the Affiliated Drum Tower Hospital of Nanjing University Medi- cal School, Nanjing 210008, China）
Objective To study the direct anti - tumor effects of the Mycobacterium boris bacillus Calmette - Guerin （BCG） - activated dendritic cell （DC） vaccine in vitro and in vivo. Methods DCs were harvested from bone marrow of C57BL/6 mice and cultured with BCG to stimulate maturation. Activated DCs were evaluated by flow cytometry （FCM）. Activated DCs were co - cultured with the pancreatic tumor cell line, Pane02, and cytotoxic response was measured by the CCK - 8 kit. The frequency of activated DCs expressing tumor necrosis factor - related apoptosis - inducing ligand （TRAIL） was measured by FCM, and TRAIL mRNA levels were detected by real - time PCR. C57BL/6 mice were implanted with Pane02 tumor cells by subcutaneous inoculation, and on the seventh day were randomly divided into three groups： untreated control, injected with saline; DC vaccine -
ISSN:1001-5256