利用EMS进行茄子种质资源创新

【目的】利用EMS诱变进行茄子种质资源创新,为茄子新品种选育及其相关基因功能研究提供良好材料。【方法】在25℃下利用不同浓度（0、0.5%、1.0%、1.5%和2.0%）的EMS溶液诱变处理茄子种子,筛选出适宜的EMS诱变浓度;然后以适宜浓度的EMS诱变液处理茄子种子,经过催芽后播种、定植,在植株的整个生育期进行相关性状调查。【结果】以1.0%EMS诱变处理18 h后,茄子种子的发芽率可达80%,适宜构建茄子突变体库。20 g茄子种子经1.0%EMS诱变处理18 h后催芽播种,最终获得984份M1代突变材料,且这些突变材料在长势、叶片形状、叶片颜色、分枝数、果实长度、果实颜色及雄性不育等方面表...

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Published in南方农业学报 Vol. 47; no. 8; pp. 1247 - 1253
Main Author 肖熙鸥 林文秋 李威 刘国强 张晓虹 吕玲玲
Format Journal Article
LanguageChinese
Published 中国热带农业科学院南亚热带作物研究所/湛江市热带作物遗传改良重点实验室,广东湛江,524091%广东海洋大学,广东湛江,524091 2016
Subjects
EMS诱变
创新
性状突变
种质资源
茄子
innovation
EMS诱变
性状突变
种质资源
germplasm resource
trait mutation
茄子
eggplant
创新
EMS mutation
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Summary:【目的】利用EMS诱变进行茄子种质资源创新,为茄子新品种选育及其相关基因功能研究提供良好材料。【方法】在25℃下利用不同浓度（0、0.5%、1.0%、1.5%和2.0%）的EMS溶液诱变处理茄子种子,筛选出适宜的EMS诱变浓度;然后以适宜浓度的EMS诱变液处理茄子种子,经过催芽后播种、定植,在植株的整个生育期进行相关性状调查。【结果】以1.0%EMS诱变处理18 h后,茄子种子的发芽率可达80%,适宜构建茄子突变体库。20 g茄子种子经1.0%EMS诱变处理18 h后催芽播种,最终获得984份M1代突变材料,且这些突变材料在长势、叶片形状、叶片颜色、分枝数、果实长度、果实颜色及雄性不育等方面表现出丰富的变异。【结论】经EMS诱变处理获得的M1代茄子表现出丰富的变异,可为其新品种选育及基因功能研究提供良好材料。
Bibliography:45-1381/S
[Objective]In order to provide fine resources for breeding of new eggplant variety and research of its gene function, the germplasm resources of eggplant were innovated by EMS mutagenesis. [Method]Under 25 ℃ condition, the eggplant seeds was mutagenized by different concentrations of EMS（0%, 0.5%, 1.0%, 1.5% and 2.0%）, respectively, so as to select optimal EMS concentration. Then the eggplant seed was mntagenized by EMS. After accelerating germination, the seedlings were transplanted to field. The mutant traits were investigated during growth period. [Result]After being mu- tagenized by 1.0% EMS for 18 h, the seed germination rate was 80%, which indicated that 1% EMS was suitable for con- struction of EMS mutants library. A total of 984 Ml generation mutants were gained after 20 g eggplant seeds was mutage- nized by 1.0% EMS. There were remarkable variations in plant growth, leaf shape, leaf color, fruit length, fruit color, fruit-bearing character and male sterile of these mutants. [Conclusion ]The
ISSN:2095-1191
DOI:10.3969/j:issn.2095-1191.2016.08.1247