广西水稻线虫的分离鉴定

【目的】明确广西部分稻区水稻长势差、叶尖端扭曲成灰白色干尖的原因,为生产上该病害的治理提供理论依据。【方法】通过直接浸泡分离法和改良贝曼漏斗法分离线虫,应用形态学和分子生物学手段相结合的方法对线虫进行鉴定。【结果】从水稻稻穗和稻秆中分离到一种线虫,形态鉴定显示,热杀死后雌虫唇区扩张,缢缩明显,口针较细,口针基部球稍膨大,中食道球长圆形,约占体宽的3/4,峡部细,食道腺背面覆盖肠,雌虫阴门位于虫体中后部,卵巢1个;用特异引物对其进行基因序列扩增,得到一条约300 bp的片段,确定造成广西部分水稻叶尖端干尖症状的病原为水稻干尖线虫。【结论】水稻干尖线虫在广西有分布和危害,应采取措施避免其扩散蔓延。...

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Published in南方农业学报 Vol. 45; no. 2; pp. 218 - 221
Main Author 黄金玲 陆秀红 万宇力 刘志明
Format Journal Article
LanguageChinese
Published 广西作物病虫害生物学重点实验室,南宁530007%广西农业科学院植物保护研究所,南宁530007 2014
广西农业科学院植物保护研究所,南宁530007
广西大学农学院,南宁530005
广西作物病虫害生物学重点实验室,南宁530007%广西大学农学院,南宁,530005
Subjects
广西
形态特征
水稻
线虫
鉴定
线虫
nematode
identification
鉴定
形态特征
Guangxi
水稻
morphological characteristic
广西
rice
Online AccessGet full text
ISSN2095-1191
DOI10.3969/j:issn.2095-1191.2014.2.218

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Summary:【目的】明确广西部分稻区水稻长势差、叶尖端扭曲成灰白色干尖的原因,为生产上该病害的治理提供理论依据。【方法】通过直接浸泡分离法和改良贝曼漏斗法分离线虫,应用形态学和分子生物学手段相结合的方法对线虫进行鉴定。【结果】从水稻稻穗和稻秆中分离到一种线虫,形态鉴定显示,热杀死后雌虫唇区扩张,缢缩明显,口针较细,口针基部球稍膨大,中食道球长圆形,约占体宽的3/4,峡部细,食道腺背面覆盖肠,雌虫阴门位于虫体中后部,卵巢1个;用特异引物对其进行基因序列扩增,得到一条约300 bp的片段,确定造成广西部分水稻叶尖端干尖症状的病原为水稻干尖线虫。【结论】水稻干尖线虫在广西有分布和危害,应采取措施避免其扩散蔓延。
Bibliography:45-1381/S
rice;nematode;identification;morphological characteristic;Guangxi
HUANG Jin-ling,LU Xiu-hong,,WAN Yu-li,LIU Zhi-ming,,（1 Plant Protection Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China; 2 Agricultural College, Guangxi University, Nanning 530005, China; 3Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests, Nanning 530007, China）
Objective】The reasons for leaf white-tip symptom in some rice cultivars in field of Guangxi were ascertained to provide theoretical references for its control in production. 【Method】Rice samples were extracted for nematodes by using immersion method and Baermann funnel techniques. Nematodes in rice were identified based on morphological and molecular methods. 【Result】A kind of nematode Aphelenchoides was isolated from rice. The morphological characters were as follows. Lip region of female was slightly expanded and wider than body at lip base. Stylet was slender. The stylet base bulb was bigger and oesophageal bulb was ovale
ISSN:2095-1191
DOI:10.3969/j:issn.2095-1191.2014.2.218