毛白杨AP2/ERF类转录因子PtoSHN的克隆及分析

AP2/ERFs是一个庞大的转录因子家族,在植物的生长发育过程中发挥着重要作用.以毛白杨（Populus tomentosa）为材料,克隆了毛白杨AP2/ERF类转录因子PtoSHN的编码区序列.组织表达特异性结果显示,PtoSHN基因在毛白杨茎中高水平表达.系统进化分析表明,PtoSHN与拟南芥AtSHN2和AtSHN3相似度较高,其中AtSHN2已被证明参与了植物次生壁的合成调控.通过亚细胞定位预测,PtoSHN定位于细胞核内.亚细胞定位结果表明,PtoSHN定位在核内.表明已成功克隆到毛白杨SHN基因,该基因可能在林木次生生长过程中起调控作用....

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Published in广东农业科学 Vol. 41; no. 17; pp. 140 - 144
Main Author 曹凌燕 陈亚娟 王宏芝 张杰伟 丁莉萍 魏建华
Format Journal Article
LanguageChinese
Published 北京农学院植物科学技术学院,北京 102206 2014
北京农业生物技术研究中心,北京100097%北京农业生物技术研究中心,北京,100097
Subjects
SHN
亚细胞定位
次生生长
毛白杨
SHN
毛白杨
亚细胞定位
次生生长
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Summary:AP2/ERFs是一个庞大的转录因子家族,在植物的生长发育过程中发挥着重要作用.以毛白杨（Populus tomentosa）为材料,克隆了毛白杨AP2/ERF类转录因子PtoSHN的编码区序列.组织表达特异性结果显示,PtoSHN基因在毛白杨茎中高水平表达.系统进化分析表明,PtoSHN与拟南芥AtSHN2和AtSHN3相似度较高,其中AtSHN2已被证明参与了植物次生壁的合成调控.通过亚细胞定位预测,PtoSHN定位于细胞核内.亚细胞定位结果表明,PtoSHN定位在核内.表明已成功克隆到毛白杨SHN基因,该基因可能在林木次生生长过程中起调控作用.
Bibliography:44-1267/S
CAO Ling-yan ,CHEN Ya-juan, WANG Hong-zhi ,ZHANG Jie-wei, DING Li-ping ,WEI Jian-hua（1.Plant Science and Technology College, Beijing University of Agriculture, Beijing 102206, China; 2. Beijing Agro-Biotechnology Research Center, Beijing 100097, China）
Populus tomentosa; SHN; secondary growth; subcellular localization
AP2/ERF is a large family of transcription factors in plants, which has great effect on plant growth and physiology. The full-length coding sequence of transcription factor SHINE （SHN） from Populus tomentosa was cloned. Real-time PCR results showed that PtoSHN expressed in various organs in poplar, especially in the stem. Phylogenetic tree proved that PtoSHN shared a high similarity with AtSHN2 and AtSHN3 from Arabidopsis thaliana. The AtSHN2 has proved to be involved in plant secondary wall synthesis regulation. PtoSHN located in the nucleus, which was predicted by bioinformational software CELLO v.2.5, and then testified by subcellular localization. Therefore, we successfully cloned SHN
ISSN:1004-874X