砷胁迫对丛枝菌根接种玉米叶片蛋白表达谱的影响

本研究采用蛋白质组学方法,研究了砷胁迫后丛枝菌根真菌接种玉米叶片蛋白表达谱的变化。以玉米叶片为实验材料,运用双向凝胶电泳技术（2-DE）对砷胁迫后丛枝菌根真菌接种玉米叶片差异表达蛋白进行分离,并通过质谱技术和搜索NCBInr数据库对差异蛋白进行鉴定。结果显示,砷胁迫后菌根接种玉米叶片中有18个蛋白差异表达,其中有6个蛋白被成功鉴定,这些蛋白包括protein phosphatase 2C、ornithine carbamoyltransferase、PEP carboxylase、两个ATP synthase CF1 beta subunit以及一个假设蛋白。该研究有助于从蛋白质水平上理解砷胁...

Full description

Saved in:
Bibliographic Details
Published in西南农业学报 Vol. 27; no. 6; pp. 2370 - 2373
Main Author 周权男 杨礼富 王真辉
Format Journal Article
LanguageChinese
Published 中国热带农业科学院橡胶研究所/农业部儋州热带作物科学观测实验站,海南儋州,571737 2014
Subjects
丛枝菌根真菌
叶片
玉米
砷胁迫
蛋白
质谱
stress
丛技菌根真菌
蛋白
玉米
mycorrhizal
Proteins
Mass
Leaves
砷胁迫
As
fungi
Zea
质谱
spectrometry
AMF
叶片
mays
Abuscular
Online AccessGet full text
ISSN1001-4829
DOI10.16213/j.cnki.scjas.2014.06.021

Cover

More Information
Summary:本研究采用蛋白质组学方法,研究了砷胁迫后丛枝菌根真菌接种玉米叶片蛋白表达谱的变化。以玉米叶片为实验材料,运用双向凝胶电泳技术（2-DE）对砷胁迫后丛枝菌根真菌接种玉米叶片差异表达蛋白进行分离,并通过质谱技术和搜索NCBInr数据库对差异蛋白进行鉴定。结果显示,砷胁迫后菌根接种玉米叶片中有18个蛋白差异表达,其中有6个蛋白被成功鉴定,这些蛋白包括protein phosphatase 2C、ornithine carbamoyltransferase、PEP carboxylase、两个ATP synthase CF1 beta subunit以及一个假设蛋白。该研究有助于从蛋白质水平上理解砷胁迫对菌根接种的影响以及菌根植物的抗砷作用机制。
Bibliography:51-1213/S
Zea mays; Leaves; As stress; Abuscular mycorrhizal fungi（AMF）; Proteins; Mass spectrometry
ZHOU Quan-nan,YANG Li-fu,WANG Zhen-hui（Rubber Research Institute, Chinese Academy of Tropical Agricultural Scienees/Danzhou Investigation ＆ Experiment Station of Tropical Crops, Ministry of Agriculture, Hainan Danzhou 571737, China）
The change of protein expression profiles from maize leaves of abuscular mycorrhizal fungi（ AMF） inoculation under arsenic（ As）stress was studied by the method of proteomics. The maize leaves were used as the experimental materials. The differentially expressed proteins in the maize leaves of AMF inoculation after As stress were separated by 2-DE and identified by mass spectrometry and searching for NCBInr database. The results showed that 18 proteins in the maize leaf differentially expressed. Of them,6 proteins were successfully identified,including protein phosphatase 2C,ornithine carbamoyltransferase,PEP carboxylase,two ATP synthase CF1 beta subunits and one hypothetical protein.
ISSN:1001-4829
DOI:10.16213/j.cnki.scjas.2014.06.021